期刊
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
卷 29, 期 37, 页码 55639-55650出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s11356-022-19701-1
关键词
Mono(2-ethylhexyl) phthalate; Lipid accumulation; Reproduction; Nontarget metabolomics; Ecdysone receptor; D; magna
资金
- National Research Council of Science and Technology (NST) - Korean government (MSIP) [CAP-17-01]
- Korea Environmental Industry & Technology Institute (KEITI) through the Core Technology Development Project for Environmental Diseases Prevention and Management - Korea Ministry of Environment (MOE) [2021003310001]
The effects of MEHP exposure on Daphnia magna were investigated, revealing that MEHP exposure increased lipid droplet numbers and the reproduction rate of adult female daphnids. Furthermore, MEHP exposure enhanced lipid metabolism and altered the expression levels of ecdysone receptor and vitellogenin.
Mono(2-ethylhexyl) phthalate (MEHP) is a primary metabolite of di(2-ethylhexyl) phthalate (DEHP), which is widely used in industry as a plasticizer. Both DEHP and MEHP have been identified as endocrine disruptors affecting reproduction systems in natural aquatic environments. However, the effects of MEHP exposure on aquatic invertebrates such as Daphnia magna are still poorly understood. In the present study, lipid alterations caused by MEHP in D. magna were identified by analyzing lipid accumulation and nontarget metabolomics. In addition, reproductive endpoints were investigated. MEHP exposure under any conditions upto 2 mg/L was not associated with mortality of D. magna; yet, the number of lipid droplets and the adult female daphnids reproduction rates increased after 96 h of exposure and 21 days of exposure, respectively. MEHP also enhanced lipid metabolism, as evident from 283 potential lipid metabolites, including glycerolipids, glycerophospholipids, and sphingolipids, identified following 48 h of exposure. The MEHP-treated group exhibited significantly higher ecdysone receptor (EcR) and vitellogenin 2 (Vtg2) expression levels at 6 and 24 h. At 48 h, EcR and Vtg2 expression levels were downregulated in the 1 and 2 mg/L MEHP exposure groups. Our data reveal that the EcR pathway changes over MEHP exposure could be associated with lipid accumulation, owing to increased lipid levels and the subsequent increase in the reproduction of MEHP-exposed D. magna.
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