期刊
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
卷 30, 期 15, 页码 42587-42595出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s11356-022-19661-6
关键词
Spodoptera frugiperda; Azadirachtin; Fat body; RNA-seq; Detoxification enzyme genes
This study investigates the effects of azadirachtin on the structure of fat bodies and detoxification-related gene expression in Spodoptera frugiperda larvae. The results suggest that azadirachtin can disrupt the fat body structure and alter the expression levels of detoxification-related genes.
The fall armyworm, Spodoptera frugiperda, has become a worldwide pest and threatens world food production. A previous study indicated that azadirachtin, the most effective botanical insecticide for S. frugiperda, inhibits larval growth of the insect. The effect of azadirachtin on the tissues of the larvae, however, remains to be determined. In this study, the effects of azadirachtin on the structure of fat bodies were analyzed. Comparative transcriptomic analysis was conducted between controls and samples treated with 0.1 mu g/g azadirachtin for 7 days to explore potential relevant mechanisms. The expression of 5356 genes was significantly affected after azadirachtin treatment, with 3020 up-regulated and 2336 down-regulated. Among them, 137 encode detoxification enzymes, including 53 P450s, 20 GSTs, 27 CarEs, 16 UGTs, and 12 ABC transporters. Our results indicated that azadirachtin could destroy fat body structure and change the mRNA levels of detoxification-related genes. The up-regulated genes encoding detoxification enzymes might be related to detoxifying azadirachtin. Our results elucidate a preliminary mechanism of azadirachtin detoxification in the fat bodies of S. frugiperda larvae.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据