期刊
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
卷 29, 期 48, 页码 72628-72638出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s11356-022-21029-9
关键词
Antifungal lipopeptide; Waste substrate; Biotransformation; Bacillus species; Extracellular enzyme; Co-culture
资金
- National Key R&D Program of China [2018YFA0902200]
- National Natural Science Foundation of China [21878224]
The efficiency of producing the broad-spectrum antifungal compound iturin A from food waste can be increased by constructing artificial bacterial consortia incorporating Bacillus amyloliquefaciens and recombinant Bacillus subtilis.
Food waste is a cheap and abundant organic resource that can be used as a substrate for the production of the broad-spectrum antifungal compound iturin A. To increase the efficiency of food waste biotransformation, different artificial consortia incorporating the iturin A producer Bacillus amyloliquefaciens HM618 together with engineered Bacillus subtilis WB800N producing lipase or amylase were constructed. The results showed that recombinant B. subtilis WB-A13 had the highest amylase activity of 23406.4 U/mL, and that the lipase activity of recombinant B. subtilis WB-L01 was 57.5 U/mL. When strain HM618 was co-cultured with strain WB-A14, the higher yield of iturin A reached to 7.66 mg/L, representing a 32.9% increase compared to the pure culture of strain HM618. In the three-strain consortium comprising strains HM618, WB-L02, and WB-A14 with initial OD600 values of 0.2, 0.15, and 0.15, respectively, the yield of iturin A reached 8.12 mg/L, which was 38.6% higher than the control. Taken together, artificial consortia of B. amyloliquefaciens and recombinant B. subtilis can produce an increased yield of iturin A, which provides a new strategy for the valorization of food waste.
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