期刊
ENVIRONMENTAL SCIENCE & TECHNOLOGY
卷 56, 期 12, 页码 8875-8884出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.est.2c00974
关键词
SARS-CoV-2; primer-probe sets; RT-qPCR; sewage surveillance; public health
资金
- Health and Medical Research Fund, Food and Health Bureau, The Government of the Hong Kong Special Administrative Region (SAR), China [COVID190209, COVID190205]
Sewage surveillance is a valuable supplementary tool for COVID-19 control, and the interpretation of sewage data is crucial. This study compared the performance of seven commonly used primer-probe sets in RT-qPCR and evaluated their usefulness in a sewage surveillance program in Hong Kong. The results showed that the N1 primer-probe set was the best in detecting signals from new cases and convalescent patients in the community.
Sewage surveillance is increasingly employed as a supplementary tool for COVID-19 control. Experiences learnt from large-scale trials could guide better interpretation of the sewage data for public health interventions. Here, we compared the performance of seven commonly used primer-probe sets in RT-qPCR and evaluated the usefulness in the sewage surveillance program in Hong Kong. All selected primer-probe sets reliably detected SARS-CoV-2 in pure water at 7 copies per mu L. Sewage matrix did not influence RT-qPCR determination of SARS-CoV-2 concentrated from a small-volume sewage (30 mL) but introduced inhibitory impacts on a large-volume sewage (920 mL) with a.Ct of 0.2-10.8. Diagnostic performance evaluation in finding COVID-19 cases showed that N1 was the best single primer-probe set, while the ORF1ab set is not recommended. Sewage surveillance using the N1 set for over 3200 samples effectively caught the outbreak trend and, importantly, had a 56% sensitivity and a 96% specificity in uncovering the signal sources from new cases and/or convalescent patients in the community. Our study paves the way for selecting detection primer-probe sets in wider applications in responding to the COVID-19 pandemic.
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