Microalgae tolerant of boron stress and bioresources accumulation during the boron removal process

Boron (B) industry and consuming produce large amounts of B-containing wastewater. Low tolerance of microorganisms and plants resulted in the biological removal of B was limited. Microalgae show high adaptability in adverse environments. Whether microalgae able to be utilized in B removal meanwhile produce bioresources, and the B tolerant mechanisms and regulation pathway of microalgae are unclear. In this study, the cell growth, B removal, and lipid/starch production of Chlorella regularis under different levels of B stress (0.5, 10, 25, and 50 mg/L) were examined. The mechanisms of signal perception and response were explored by transcriptome and network analysis. Microalgae tolerated 25 mg/L high B stress, cell growth showed no decline and biomass reach up to 4.5 g/L. Microalgae took in B with 3.35 mg/g and bonded them to protein and carbon components in cells, the B removal capability was higher than some special adsorbents. Microalgae produced 188.65 mg/(L center dot d) lipids and 305.35 mg/(L center dot d) starch. The mitogen-activated protein-kinase signaling pathway was involved in the B tolerance of microalgae and regulated B efflux, glycolysis, and lipid/starch accumulation to relieve B stress. This study provides potential biological technique for B removal in wastewater and promotes new insight into signal role in toxic pollutants biological treatment.

Automated summary

This study found that Chlorella regularis exhibited high tolerance to high concentrations of boron (B), maintaining cell growth and producing a high biomass. The microalgae removed B by absorbing it and binding it to protein and carbon components in the cells, with a removal capability exceeding that of some special adsorbents. Furthermore, the mitogen-activated protein-kinase signaling pathway was involved in the B tolerance of microalgae, regulating B efflux, glycolysis, and lipid/starch accumulation.