Presence of nano-sized mercury-containing particles in seafoods, and an estimate of dietary exposure

The toxicity of nano-sized particles of mercury (NP-Hg), which are thought to be generated during the detoxification of methyl mercury (MeHg), may differ from that of MeHg, elemental Hg (Hg0), and inorganic Hg (I-Hg). From a human health perspective, it is important to evaluate the presence of NP-Hg in seafoods. We investigated the in vivo formation of NP-Hg in fish and shellfish, which are the main sources of Hg exposure in humans. NP-Hg was measured in 90 fish samples with single-particle inductively coupled plasma mass spectrometry (spICP-MS) after enzyme degradation with pancreatin and lipase. In addition to NP-Hg, total Hg (T-Hg), MeHg, and selenium (Se) concentrations were evaluated. Transient Hg signals were detected as nanoparticles from almost all samples by using spICP-MS. Higher particle number concentrations (CPN) were observed in the tuna-swordfish group than in the shellfish group (17.7 x 10(7) vs. 1.2 x 10(6) particles/g, respectively). Although the CPN and maximum particle mass increased significantly with increasing T-Hg concentration, the increase in CPN was greater than those in maximum particle mass. Assuming that the NP-Hg detected was HgSe (tiemannite) and spherical based on previous reports, the maximum particle diameter was estimated to be 89 nm. The mean dietary exposures to NP-Hg, T-Hg, and MeHg were estimated to be 0.067, 5.75, and 5.32 mu g/person per day, respectively. Generation of NP-Hg was inferred to be widespread in marine animals, with a preferential increase in the number of particles rather than an increase in particle size. The mean dietary exposure to NP-Hg in Japanese people was estimated to be 1.2 ng/kg body weight (BW) per day. Compared to PTWI of 4 mu g/kg BW per week (0.57 mu g/kg BW per day) derived by JECFA (2011), the health risk from redissolved I-Hg from NP-Hg is small.

Automated summary

This study investigated the formation of nano-sized particles of mercury (NP-Hg) in fish and shellfish, which are the main sources of human exposure to mercury. The results showed that NP-Hg was present in almost all fish samples, with higher concentrations observed in tuna and swordfish. The study also estimated the dietary exposure to NP-Hg in Japanese people and found it to be relatively low, indicating a small health risk from redissolved inorganic mercury.