Toxicokinetics of metals in the soil invertebrate Enchytraeus crypticus exposed to field-contaminated soils from a mining area

Toxicokinetics may help assessing the risk of metal-contaminated soils by quantifying the development of internal metal concentrations in organisms over time. This study assessed the toxicokinetics in Enchytraeus crypticus of non-essential (Pb and Cd) and essential elements (Zn and Cu) in metal-contaminated field soils from a mining area, containing 3.49-24.3 mg Cd/kg dry soil, 433-1416 mg Pb/kg dry soil, 15.7-44.9 mg Cu/kg dry soil and 1718-6050 mg Zn/kg dry soil. Three different uptake-elimination patterns in E. crypticus were found. Both essential elements (Zn and Cu) showed fast increasing internal concentrations reaching equilibrium within 2 d in the uptake phase, without hardly any elimination after transfer to clean soil. The non-essential Cd showed a slow linear accumulation and excretion with body concentrations not reaching steady state within 21 d. Internal Pb concentrations, however, reached equilibrium within 7 d in the uptake phase. Longer exposure times in ecotoxicological tests, therefore, are required for elements like Cd. Porewater pH and dissolved organic carbon (DOC) levels were the dominant factors controlling Cd uptake from the test soils. The 21-d body Cd and Pb concentrations were best explained from 0.01 M CaCl2-extractable soil concentrations. Steady-state Cu and Zn body concentrations were independent of soil exposure concentrations. Bioaccumulation factors (BAF) were low for Pb (<0.1 kg(soil)/kg(worm)), but high for Cd at 1.78-24.3 kg(soil)/kg(worm), suggesting a potential risk of Cd biomagnification in the terrestrial food chain of the mining area ecosystem.

Automated summary

Toxicokinetics is a useful tool for assessing the risk of metal-contaminated soils. This study investigated the toxicokinetics of non-essential and essential elements in Enchytraeus crypticus exposed to metal-contaminated soils from a mining area. The study found different uptake-elimination patterns for different elements and suggested that longer exposure times are needed for elements like Cd. pH and dissolved organic carbon levels were found to be key factors controlling Cd uptake. The study also highlighted the potential risk of Cd biomagnification in the food chain of the mining area ecosystem.