期刊
ENGINEERING IN LIFE SCIENCES
卷 23, 期 1, 页码 -出版社
WILEY
DOI: 10.1002/elsc.202100151
关键词
high-throughput; microtiter plate; online monitoring; pigmentation; Streptomyces coelicolor
In this study, high-throughput online monitoring was used to gain a deep understanding of the cultivation process of the model organism Streptomyces coelicolor A3(2). By measuring the oxygen transfer rate and autofluorescence online, valuable information about nutrient availability and pigment product formation patterns can be obtained. The study identified the filling volume of the cultivation vessel as a factor influencing pigment formation.
Streptomyces species are intensively studied for their ability to produce a variety of natural products. However, conditions influencing and leading to product formation are often not completely recognized. Therefore, in this study, high-throughput online monitoring is presented as a powerful tool to gain in-depth understanding of the cultivation of the model organism Streptomyces coelicolor A3(2). Through online measurements of oxygen transfer rate and autofluorescence, valuable information about availability of nutrients and product formation patterns of the pigments actinorhodin and undecylprodigiosin can be obtained and explained. Therefore, it is possible to determine the onset of pigmentation and to study in detail the influencing factors thereof. One factor identified in this study is the filling volume of the cultivation vessel. Slight variations led to varying pigmentation levels. By combining optical and metabolic online monitoring techniques, the correlation of the filling volume with pigmentation could be explained as a result of different growth trajectories caused by varying specific power inputs and their influence on the pellet formation of the filamentous system. Finally, experiments with the addition of supernatant from unpigmented and pigmented cultures could highlight the applicability of the presented approach to study quorum sensing and cell-cell interaction.
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