期刊
EMBO REPORTS
卷 23, 期 7, 页码 -出版社
WILEY
DOI: 10.15252/embr.202153956
关键词
antibodies; B cells; class switch; MACACS; membrane antigen
资金
- Swiss National Science Foundation [189043, 189151]
- Universitat Basel
In this study, we isolated spike-protein-specific B cells and produced recombinant antibodies of IgM, IgG, and IgA classes. Two IgM antibodies demonstrated potent virus neutralization at picomolar concentrations, but this potency was lost when switched to IgG. Decreased avidity alone cannot explain the loss of potency.
To investigate the class-dependent properties of anti-viral IgM antibodies, we use membrane antigen capture activated cell sorting to isolate spike-protein-specific B cells from donors recently infected with SARS-CoV-2, allowing production of recombinant antibodies. We isolate 20, spike-protein-specific antibodies of classes IgM, IgG, and IgA, none of which shows any antigen-independent binding to human cells. Two antibodies of class IgM mediate virus neutralization at picomolar concentrations, but this potency is lost following artificial switch to IgG. Although, as expected, the IgG versions of the antibodies appear to have lower avidity than their IgM parents, this is not sufficient to explain the loss of potency.
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