Long-distance mobile mRNA CAX3 modulates iron uptake and zinc compartmentalization

Iron deficiency in plants can lead to excessive absorption of zinc; however, important details of this mechanism have yet to be elucidated. Here, we report that MdCAX3 mRNA is transported from the leaf to the root, and that MdCAX3 is then activated by MdCXIP1. Suppression of MdCAX3 expression leads to an increase in the root apoplastic pH, which is associated with the iron deficiency response. Notably, overexpression of MdCAX3 does not affect the apoplastic pH in a MdCXIP1 loss-of-function Malus baccata (Mb) mutant that has a deletion in the MdCXIP1 promoter. This deletion in Mb weakens MdCXIP1 expression. Co-expression of MdCAX3 and MdCXIP1 in Mb causes a decrease in the root apoplastic pH. Furthermore, suppressing MdCAX3 in Malus significantly reduces zinc vacuole compartmentalization. We also show that MdCAX3 activated by MdCXIP1 is not only involved in iron uptake, but also in regulating zinc detoxification by compartmentalizing zinc in vacuoles to avoid iron starvation-induced zinc toxicity. Thus, mobile MdCAX3 mRNA is involved in the regulation of iron and zinc homeostasis in response to iron starvation.

Automated summary

Iron deficiency in plants can lead to excessive absorption of zinc. This study reveals the mechanism of MdCAX3 mRNA transport from the leaf to the root, and its activation by MdCXIP1. MdCAX3 suppression increases root apoplastic pH associated with the iron deficiency response. Overexpression of MdCAX3 affects the apoplastic pH only in the presence of MdCXIP1. Furthermore, MdCAX3 is involved in zinc compartmentalization and plays a role in iron and zinc homeostasis.