In vitro characterisation of murine pre-adipose nucleated cells reveals electrophysiological cycles associated with biological clocks

Adipocytes are energy stores of the body which also play a role in physiological regulation and homeostasis through their endocrine activity. Adipocyte circadian clocks drive rhythms in gene expression, and dysregulation of these circadian rhythms associates with pathological conditions such as diabetes. However, although the role of circadian rhythms in adipose cells and related tissues has been studied from phsyiological and molecular perspectives, they have not yet been explored from an electrical perspective. Research into electro-chronobiology has revealed that electrical properties have important roles in peripheral clock regulation independently of transcription-translation feedback loops. We have used dielectrophoresis to study electrophysiological rhythms in pre-adipocytes - representing an adipocyte precursor and nucleated cell-based model, using serum shocking as the cellular method of clock entrainment. The results revealed significant electrophysiological rhythms, culminating in circadian (ca. 24 hourly) cycles in effective membrane capacitance and radius properties, whereas effective membrane conductance was observed to express ultradian (ca. 14 hourly) rhythms. These data shed new light into pre-adipocyte electrical behaviour and present a potential target for understanding and manipulation of metabolic physiology.

Automated summary

Research reveals that the electrical properties in adipocytes play a crucial role in peripheral clock regulation, independent of transcription-translation feedback loops. Using dielectrophoresis, significant electrophysiological rhythms were observed in pre-adipocytes, with circadian (around 24 hours) cycles in membrane capacitance and radius properties, and ultradian (around 14 hours) rhythms in membrane conductance.