期刊
ELECTROCHIMICA ACTA
卷 415, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.electacta.2022.140240
关键词
Electrochemiluminescence imaging; Cell analysis; Cell adhesion; Vascular endothelial cell; Tube formation
资金
- Japan Society for the Promotion of Science [20H00619, 18H01840, 18H01999, 21H01957, 21H03803, 19K20658, 20J21401]
- Shimadzu Science Foundation
- Electrochemical Society of Japan, JST COI [JPMJCE1303]
- Japan Association for Chemical Innovation
- Program for Creation of Interdisciplinary Research from Frontier Research Institute for Interdisciplinary Sciences, Tohoku University
- System Design of Inclusive Society with Infectious Diseases
- Grants-in-Aid for Scientific Research [21H03803, 21H01957, 20H00619, 20J21401, 19K20658, 18H01840, 18H01999] Funding Source: KAKEN
This study presents a method for electrochemiluminescence (ECL) imaging to visualize cellular adhesion on hydrogel scaffolds. By culturing human umbilical vein endothelial cells on a Matrigel-coated indium tin oxide (ITO) electrode and using an ECL solution and specific voltage, ECL signals were generated, allowing for imaging of cellular adhesion.
This study describes a method for electrochemiluminescence (ECL) imaging of cellular adhesion in vascular endothelial cells during tube formation on hydrogel scaffolds. Human umbilical vein endothelial cells were cultured on a Matrigel-coated indium tin oxide (ITO) electrode to facilitate tubule growth. Once these tubes were formed, the culture medium was changed to an ECL solution containing [Ru(bpy)3]2+ and tripropylamine and then subjected to 1.2 V vs Ag/AgCl (sat. KCl) to produce ECL signals at the ITO electrode. This allowed for ECL imaging of cellular adhesion because these ECL signals were blocked in areas typified by strong cell-cell and cell-substrate adhesion. The imaging showed that there were two types of cellular adhesion during tube formation. To the best of our knowledge, this is the first report on ECL imaging for adhesion between cells and between cells and substrates during tube formation on hydrogel scaffolds. We believe that this ECL imaging technique will be useful for the evaluation of organs-on-a-chip and for drug screening targeting cell-cell and cell-substrate interactions in the future.
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