Assembling a Low-volume Biofuel Cell on a Screen-printed Electrode for Glucose Sensing

An enzymatic biofuel cell is integrated on a screen-printed electrode as a basis for a self-powered biosensor. A glucose/O-2 biofuel cell consisting of a pyrroloquinoline quinone-dependent glucose dehydrogenase embedded within an Os-complex modified redox polymer bioanode to oxidize glucose and a non-limiting bilirubin oxidase-based gas diffusion biocathode in the direct-electron transfer regime for the reduction of O-2 showed a glucose-dependent current and power output. For full integration on a single screen-printed electrode, a miniaturized agar salt bridge was introduced between the two bioelectrodes to ensure operation of the assembly in a two-compartment configuration with each electrode operating at optimal conditions.

Automated summary

In this study, an enzymatic biofuel cell integrated on a screen-printed electrode was developed as a self-powered biosensor. By introducing a miniaturized agar salt bridge, full integration of two bioelectrodes was achieved, allowing the assembly to operate in a two-compartment configuration with optimal conditions for each electrode.