期刊
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
卷 238, 期 -, 页码 -出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ecoenv.2022.113605
关键词
Sulfometuron-methyl; High-throughput sequencing; Phylogenetic molecular ecological networks; Ralstonia sp. JM-1; Immobilization; Biodegradation
资金
- Natural Science Foundation of Henan Province [212300410215]
- National Natural Science Foundation of China [31900082]
- High-qualified Talents Scientific Research Startup Foundation of Nanyang Normal University [2019ZX015, 2019ZX013]
The analysis of soil bacterial community is important for utilizing soil microbial resources. The presence of sulfometuron-methyl affects the diversity, richness, and function of soil bacteria. Ralstonia is identified as a potential degrader and forms a stable relationship with indigenous microorganisms. The immobilized strains of Ralstonia show higher sulfometuron-methyl degradation efficiency compared to free state strains.
The analysis of soil bacterial community has guiding significance for fully utilization of soil microbial resources. The results of high-throughput sequencing (HTS) showed that the bacteria in the three sulfometuron-methyl contaminated soil samples were mainly composed of 677 genera, including Phenylobacterium, Bacillus, belonging to 28 phyla, including Proteobacteria, Firmicutes. The diversity and richness of bacterial community decreased with the increase in sulfometuron-methyl concentration. In addition, sulfometuron-methyl could also affect the soil bacterial function based on PICRUSt functional predictive analysis. Combined with the results of HTS and phylogenetic molecular ecological networks (pMENs), 12 genera, including Ralstonia (Pi=0.64), were identified as the key soil microflora (intra-module connectivity Zi >= 2.5 or inter-module connectivity Pi >= 0.62), and the abundance of Ralstonia significantly increased with the concentration of sulfometuron-methyl, indicating that the strains of this genus might be the potential degrading bacteria and could form a stable relationship with indigenous microorganisms. Among the isolated bacteria of genus Ralstonia, one strain, named Ralstonia sp. JM-1, was verified to possess higher sulfometuron-methyl degradation efficiency, which completely degraded 20 mg L-1 of sulfometuron-methyl within 96 h. Furthermore, the immobilized strains generated by the mixture of 2.0 g bamboo charcoal and 3.0 mL bacterial suspension for 24 h had the highest sulfometuron-methyl degradation rate than that under other conditions, and the dynamic process degrading 10-30 mg L-1 of sulfometuron-methyl conforms to the zero-order kinetic equation. The bioremediation of contaminated soil showed the immobilized strains could completely degrade sulfometuron-methyl (1.39 mg kg(-1)) in contaminated soil within 9 d, which is higher than that application of strains in the free state (74.8%). This study could provide ideas for the isolation of functional strains and a theoretical basis for the bioremediation of STM and other contaminated soils.
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