A sustainable and economic strategy to reduce risk antibiotic resistance genes during poultry manure bioconversion by black soldier fly Hermetia illucens larvae: Larval density adjustment

Black soldier fly (Hermetia illucens) larvae (BSFL) are common insects that are known for bioconversion of organic waste into a sustainable utilization resource. However, a strategy to increase antibiotic resistance gene (ARG) elimination in sustainable and economic ways through BSFL is lacking. In the present study, different larval densities were employed to assess the mcr(-1) and tetX elimination abilities, and potential mechanisms were investigated. The application and economic value of each larval density were also analyzed. The results showed that the 100 larvae cultured in 100 g of manure group had the best density because the comprehensive disadvantage evaluation ratio was the lowest (14.97%, good bioconversion manure quality, low ARG deposition risk and reasonable larvae input cost). Further investigation showed that mcr(-1) could be significantly decreased by BSFL bioconversion (4.42 x10(7) copies/g reduced to 4.79 x10(6)-2.14 x10(5) copies/g)(P<0.05); however, mcr(-1) was increasingly deposited in the larval gut with increasing larval density. The tetX abundance was stabilized by BSFL bioconversion, except that the abundance at the lowest larval density increased (1.22 x10(10) copies/g increase, 34-fold). Escherichia was the host of mcr(-1) and tetX in all samples, especially in fresh manure; Alcaligenes was the host of tetX in bioconversion manure; and the abundance of Alcaligenes was highly correlated with the pH of bioconversion manure. The pH of bioconversion manure was extremely correlated with the density of larvae. Klebsiella and Providencia were both hosts of tetX in the BSF larval gut, and Providencia was also the host of mcr(-1) in the BSF larval gut. The density of larvae influenced the bioconversion manure quality and caused the ARG host abundance to change to control the abundance of ARGs, suggesting that larval density adjustment was a useful strategy to manage the ARG risk during BSFL manure bioconversion.

Automated summary

Black soldier fly larvae have the ability to convert organic waste into a sustainable resource. This study found that a density of 100 larvae cultured in 100 g of manure resulted in the best elimination of antibiotic resistance genes (ARGs), although the deposition of ARGs in the larval gut increased with higher larval density. The adjustment of larval density can effectively manage the risk of ARGs during the bioconversion process of black soldier fly larvae manure.