期刊
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
卷 236, 期 -, 页码 -出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ecoenv.2022.113439
关键词
Chinese mitten carb; Microcystin-LR; Apoptosis; Lipid metabolism; Transcriptomic Responses
资金
- National Natural Science Foundation of China [31802347]
- Fundamental Research Funds for the Central Universities [KJQN201937]
- earmarked fund for the Jiangsu Agricultural Industry Technology System [JATS [2021] 474]
- Jiangsu Agriculture Science and Technology Innova-tion Fund [CX (20) 3109]
- Breeding and Quality Improvement of Livestock and Poultry [B21021]
Global warming increases harmful cyanobacterial blooms, and microcystin-LR (MC-LR) is a hepatotoxic agent produced during these blooms. This study injected different doses of MC-LR into the hepatopancreas of Chinese mitten crab and found that it affected gene expression, leading to triglyceride accumulation and changes in sterol metabolism, apoptosis, and actin cytoskeleton regulation.
Global warming is favouring the incidence, intensity and duration of harmful cyanobacterial blooms. Microcystin-LR (MC-LR), a hepatotoxic agent, is produced during cyanobacterial blooms. To understand the molecular mechanisms of acute hepatotoxic effect of low doses of MC-LR in crab, we examined differentially expressed genes in samples of the hepatopancreas of Chinese mitten crab (Eriocheir sinensis) collected in 48 h after injections of MC-LR at doses of 0, 25, 50, and 75 mu g/kg. The results revealed that MC-LR induced changes in corresponding gene led to the accumulation of triglycerides. MC-LR exposure affected sterol metabolism. Apoptosis-related genes such as Fas-L, Bcl-XL, Cytc, AiF, p53, PERK, calpain, CASP2, CASP7, alpha-tubulin, PARP, GF, G12, and PKC were upregulated. Conversely, expression levels of CASP10 and ASK1 were downregulated. Genes related to the regulation of actin cytoskeleton (Rho, ROCK, MLCP, MLC, PAK, and PFN) were upregulated. Further, expression levels of genes encoding fatty acid elongation-related enzymes were upregulated, but the expression of genes related to fatty acid synthesis was slightly down regulated. Taken together, these results demonstrated the hepatic toxicity and molecular mechanisms of changes in lipid metabolism, immune and apoptosis in Chinese mitten crab under the MC-LR-induced stress, which is the first report on crabs and performs a comprehensive analysis and a new insight of the molecular toxicological responses in crabs.
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