4.6 Article

Synthesis of lysozyme-reduced graphene oxide films for biosensor applications

期刊

DIAMOND AND RELATED MATERIALS
卷 126, 期 -, 页码 -

出版社

ELSEVIER SCIENCE SA
DOI: 10.1016/j.diamond.2022.109093

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Biosensor; Graphene; Reduced graphene oxide; SARS-CoV-2

资金

  1. VRS Technology and Innovation Eirelli
  2. 2MI Group

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Graphene-based materials with high surface area and structural defects have the potential for efficient interaction with biomolecules, making them promising for applications in biosensor devices.
Graphene-based materials present a high surface area and can present several structural defects or heteroatoms depending on the fabrication method. Such properties facilitate the graphene interaction with biomolecules leading to high electrochemical activity. In this context, we synthesize a high-quality dispersed reduced graphene oxide using Lysozyme as a dispersant (Ly-RGO). In this study, we have investigated the graphite oxidation-reduction fabrication process using X-ray Photoelectron Spectroscopy, X-ray Diffraction, and Raman Spectroscopy. We have used the Ly-RGO to fabricate an electrode on an electrochemical cell in which voltammograms show significant transduction of electrical signals. Finally, cyclic voltammetry and chronoamperometry measurements were performed with human saliva and SARS-CoV-2 RNA samples, to calibrate our biosensor. The results indicate that Ly-RGO has great potential in biosensor devices.

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