期刊
DEVELOPMENTAL NEUROBIOLOGY
卷 82, 期 3, 页码 245-260出版社
WILEY
DOI: 10.1002/dneu.22871
关键词
arginine methylation; brain development; Carm1; differentiation; myelination; oligodendrocyte; protein arginine methyltransferase
资金
- Osaka Medical Research Foundation for Intractable Diseases [26-2-2]
- Japan Agency for Medical Research and Development [DNW-14010]
- Kindai University [SR05]
- Japan Foundation for Applied Enzymology
- Japan Society for the Promotion of Science [19K16268, 19K06916, 16K07073]
- Grants-in-Aid for Scientific Research [19K06916, 19K16268, 16K07073] Funding Source: KAKEN
Protein arginine methylation, mediated by Carm1, plays a crucial role in regulating cellular events such as cell proliferation and differentiation. In this study, Carm1 was found to be highly expressed in oligodendrocyte lineage cells in the developing brain, and its inhibition resulted in reduced proliferation and impaired differentiation of these cells. Carm1 activity was also found to be necessary for the appropriate formation of myelin processes and myelin sheaths, suggesting its essential role in the development of oligodendrocytes.
Protein arginine methylation has been recognized as one of key posttranslational modifications for refined protein functions, mediated by protein arginine methyltransferases (Prmts). Coactivator-associated arginine methyltransferase (Carm1, also known as Prmt4) participates in various cellular events, such as cell survival, proliferation, and differentiation through its protein arginine methylation activities. Carm1 regulates cell proliferation of a neuronal cell line and is reportedly expressed in the mammalian brain. However, its detailed function in the central nervous system, particularly in glial cells, remains largely unexplored. In this study, Carm1 exhibited relatively high expression in oligodendrocyte (OL) lineage cells present in the corpus callosum of the developing brain, followed by a remarkable downregulation after active myelination. The suppression of Carm1 activity by inhibitors in isolated oligodendrocyte precursor cells (OPCs) reduced the number of Ki67-expressing and BrdU-incorporated proliferating cells. Furthermore, Carm1 inactivation attenuated OL differentiation, as determined by the expression of Plp, a reliable myelin-related marker. It also impaired the extension of OL processes, accompanied by a significant reduction in gene expression related to OL differentiation and myelination, such as Sox10, Cnp, Myrf, and Mbp. In addition, OLs co-cultured with embryonic dorsal root ganglia neurons demonstrated that Carm1 activity is required for the appropriate formation of myelin processes and myelin sheaths around neuronal axons, and the induction of the clustering of Caspr, a node of Ranvier structural molecule. Thus, we propose that Carm1 is an essential molecule for the development of OPCs and OLs during brain development.
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