The great small organisms of developmental genetics: Caenorhabditis elegans and Drosophila melanogaster

Experimental embryologists working at the turn of the 19th century suggested fundamental mechanisms of development, such as localized cytoplasmic determinants and tissue induction. However, the molecular basis underlying these processes proved intractable for a long time, despite concerted efforts in many developmental systems to isolate factors with a biological role. That road block was overcome by combining developmental biology with genetics. This powerful approach used unbiased genome-wide screens to isolate mutants with developmental defects and to thereby identify genes encoding key determinants and regulatory pathways that govern development. Two small invertebrates were the pioneers: the fruit fly Drosophila melanogaster and the nematode Caenorhabditis elegans. Their modes of development differ in many ways, but the two together led the way to unraveling the molecular mechanisms of many fundamental developmental processes. The discovery of the grand homologies between key players in development throughout the animal kingdom underscored the use-fulness of studying these small invertebrate models for animal development and even human disease. We describe developmental genetics in Drosophila and C. elegans up to the rise of genomics at the beginning of the 21st Century. Finally, we discuss themes that emerge from the histories of such distinct organisms and prospects of this approach for the future.

Automated summary

Experimental embryologists in the late 19th century proposed fundamental mechanisms of development, but the molecular basis remained elusive for a long time. By combining developmental biology with genetics, this obstacle was overcome. Fruit flies and nematodes played a pioneering role in unraveling the molecular mechanisms of developmental processes, highlighting the importance of studying these small invertebrate models for understanding animal development and human disease.