期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 129, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2022.104348
关键词
MMP25; Monocytes/macrophages; Japanese sea bass; Pro-inflammatory; Phagocytosis
资金
- Zhejiang Provincial Natural Science Foundation of China [LR18C040001, LZ18C190001]
- Program for the Natural Science Foundation of China [41776151, 31972821]
- Fundamental Research Funds for the Provincial Universities of Zhejiang [SJLZ2020002]
This study identified the cDNA sequence of MMP25 in Japanese sea bass and revealed its role in regulating inflammatory responses. LjMMP25 was found to regulate inflammatory cytokine production and promote phagocytosis and bacterial killing in monocytes/macrophages. LjMMP25 also modulated the inflammatory response by regulating NF-kappa B signaling.
Matrix metalloproteinases (MMPs) are highly expressed in leukocytes and macrophages, which play a role in the innate immune response. Here, the cDNA sequence of MMP25 from Japanese sea bass (Lateolabrax japonicus) (LjMMP25) was identified. Phylogenetic analysis revealed that LjMMP25 was most closely related to large yellow croaker MMP25. Multiple sequence alignment of LjMMP25 with MMP25 sequences from other teleosts revealed that regions of known functional importance were highly conserved. Expression analysis revealed that LjMMP25 was highly expressed in the head kidney and widely expressed in other tissues including gill, spleen, and liver. LjMMP25 was found to regulate inflammatory cytokine production and promote phagocytosis and bacterial killing in monocytes/macrophages (MO/M?). Furthermore, LjMMP25 regulated the inflammatory response by modulating NF-kappa B signaling. These findings reveal new information about the role of LjMMP25 in regulating pro inflammatory responses in this species.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据