期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 130, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2022.104360
关键词
Fenneropenaeus merguiensis; Innate immunity; Clip domain serine proteinase; Antibacterial activity
资金
- Research Institute for Health Sciences, Walailak University [WU-IRG-63-060]
- Prince of Songkla University through the Graduate School, Scholarship for PSUPh.D. Students [PSU_PHD2561-002]
- Prince of Songkla University through the Graduate School
- Prince of Songkla University [SCI6202088S]
- Prince of Songkla University through Scholarship for Thai Ph.D. Students under Thailand's Education Hub for Southern Region of ASEAN Countries [PHD/2558]
This study partially characterized the function of FmclipSP and demonstrated its association with VP28. The results indicate the importance of FmclipSP in the innate immunity of F. merguiensis.
Clip domain serine proteinases participate in invertebrate innate immunity by acting as crucial enzymes in the signaling cascade involved in shrimp immunity. To functionally characterize its role in Fenneropenaeus mer-guiensis, FmclipSP cDNA was cloned and characterized. The FmclipSP gene comprised 1353 bp with an open reading frame of 1110 bp and encoded 369 amino acids. The protein contained clip and serine protease domains. FmClipSP mRNA is highly expressed in hemocytes, and its expression was significantly upregulated by bacterial or viral pathogen challenge. Furthermore, FmClipSP recombinant protein (rFmClipSP) was produced and possessed protease activity, stimulating prophenoloxidase activity. Additionally, rFmClipSP exhibited antibac-terial activity against pathogens and nonpathogens. ELISA results demonstrated the binding ability of rFmClipSP to a recombinant protein of VP28 (rVP28). Interestingly, the binding significantly inhibited prophenoloxidase activity. Altogether, we partially characterized the function of FmclipSP and demonstrated its association with VP28. This study indicates the importance of clipSP as a component of F. merguiensis innate immunity. However, the role of clipSP in crustaceans remains unclear and requires further investigation.
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