4.7 Article

Chondroitin sulfate enhances the barrier function of basement membrane assembled by heparan sulfate

期刊

DEVELOPMENT
卷 149, 期 12, 页码 -

出版社

COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.200569

关键词

Retina; Chondroitin sulfate; Astrocyte; Basement membrane; Angiogenesis; Glycocalyx

资金

  1. National Institutes of Health (NIH) [EY017061, EY018868, EY025933, EY031210, HL107150, HL57345, EY029428, EY032966]
  2. NIH [5P30EY019007]
  3. Research to Prevent Blindness (RPB)
  4. Barbara and Donal Jonas Family Fund
  5. Jules and Doris Stein Research to Prevent Blindness Professorship

向作者/读者索取更多资源

The study demonstrates the essential role of glycosaminoglycans in retinal angiogenesis, blood vessel pruning, and establishment of the basement membrane. The absence of heparan sulfate affects VEGF signaling, disrupts retinal inner limiting membrane assembly, and increases retinal permeability.
Glycosaminoglycans are ubiquitously expressed polysaccharides that are attached to proteoglycans. Here, we showed that ablation of the heparan sulfate (HS) polymerase Ext1 in retinal progenitor cells did not affect initial progression of retinal angiogenesis, but it disrupted the pruning of blood vessels and establishment of arterioles and venules. In the absence of retinal HS, blood vessels were also vulnerable to high oxygen tension in early postnatal stages, which could be rescued by exogenous vascular endothelial growth factor (VEGF), consistent with the role of retinal HS in the fine-tuning of VEGF signaling. Furthermore, we observed that the retinal inner limiting membrane (ILM) was disrupted by deletion of Ext1 in a timing-specific manner, suggesting that retinal HS is required for the assembly but not the maintenance of the basement membrane. Lastly, we showed that further deletion of C4st1, a chondroitin sulfate (CS) sulfation enzyme, did not affect the assembly of the ILM but, when combined with Ext1 deletion, it aggravated the retinal permeability by disrupting the retinal glycocalyx. These results demonstrate an important role of CS and HS in establishing the barrier function of the extracellular matrix.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据