期刊
DEVELOPMENT
卷 149, 期 5, 页码 -出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.200026
关键词
Corticopontine topography; Layer V pyramidal neurons; Gradients of area mapping genes; Nr2f1; Conditional knockout mouse models; Thy1-eYFP-H reporter line; Pontine nuclei; 3D data analysis; 3D digital brain atlas; Anterograde fluorescent tracing
资金
- Fondation Recherche Medicale [FDT201904008137, DEQ20150331750]
- French Government (National Research Agency) [ANR-11-LABX-0028-01, ANR-15-IDEX-01]
- Region PACA/Inserm
- European Union [945539]
- Research Council of Norway [269774]
- University of Oslo
This study reveals that the expression of Nr2f1 gene in the cortical region directly affects the topographic mapping from the cortex to the pontine nuclei. Using transgenic mice and various techniques, the researchers identified that Nr2f1 expression in postmitotic neurons is crucial for controlling somatosensory topographic projections, while its expression in progenitor cells influences the ratio of different fiber types.
Axonal projections from layer V neurons of distinct neocortical areas are topographically organized into discrete clusters within the pontine nuclei during the establishment of voluntary movements. However, the molecular determinants controlling corticopontine connectivity are insufficiently understood. Here, we show that an intrinsic cortical genetic program driven by Nr2f1 graded expression is directly implicated in the organization of corticopontine topographic mapping. Transgenic mice lacking cortical expression of Nr2f1 and exhibiting areal organization defects were used as model systems to investigate the arrangement of corticopontine projections. By combining three-dimensional digital brain atlas tools, Cre-dependent mouse lines and axonal tracing, we show that Nr2f1 expression in postmitotic neurons spatially and temporally controls somatosensory topographic projections, whereas expression in progenitor cells influences the ratio between corticopontine and corticospinal fibres passing the pontine nuclei. We conclude that cortical gradients of area-patterning genes are directly implicated in the establishment of a topographic somatotopic mapping from the cortex onto pontine nuclei.
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