4.4 Review

Anti-cancer Virotherapy in Russia: Lessons from the Past, Current Challenges and Prospects for the Future

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Superior infectivity of the fiber chimeric oncolytic adenoviruses Ad5/35 and Ad5/3 over Ad5-delta-24-RGD in primary glioma cultures

Aleksei A. Stepanenko et al.

Summary: Ad5-delta-24-RGD is the most clinically advanced recombinant adenovirus for glioma therapy. Different fiber-modified rAds were constructed and compared, showing that Ad5/35-delta-24 and Ad5/3-delta-24 exhibited superior infectivity and cytolytic efficacy compared to Ad5-delta-24-RGD. The expression of adenoviral receptors/coreceptors and integrins did not predict the cytolytic efficacy of the fiber-modified rAds. CT-2A and GL261 glioma cells were identified as murine cell models for studying fiber chimeric rAds. Ad5/35-delta-24 armed with the immune costimulator OX40L demonstrated potential for glioblastoma treatment.

MOLECULAR THERAPY-ONCOLYTICS (2022)

Article Biochemistry & Molecular Biology

There is still no evidence of SARS-CoV-2 laboratory origin: Response to Segreto and Deigin (10.1002/bies.202100137)

Alexander Tyshkovskiy et al.

Summary: The causative agent of COVID-19, SARS-CoV-2, has caused over 4 million deaths worldwide. While there are claims that suggest artificial creation of the virus, existing genetic data, viral diversity, and human history point towards a natural origin. Further genetic evidence supporting an alternative theory is yet to be presented.

BIOESSAYS (2021)

Article Oncology

Multi-Omics Analysis of Glioblastoma Cells' Sensitivity to Oncolytic Viruses

Anastasiya V. Lipatova et al.

Summary: Studies have shown that interferon-induced resistance may affect the sensitivity of tumor cells to viruses, but inhibiting a single ISG may not necessarily increase sensitivity. Silencing specific genes may negatively impact the internalization of certain viruses.

CANCERS (2021)

Review Pharmacology & Pharmacy

Adenovirus Type 6: Subtle Structural Distinctions from Adenovirus Type 5 Result in Essential Differences in Properties and Perspectives for Gene Therapy

Margarita Romanenko et al.

Summary: Adenovirus type 6 (HAdV-C6) shows promise in gene therapy, especially in vaccine and anticancer drug development. Compared to HAdV-C5, HAdV-C6 differs structurally and immunogenically, enabling evasion of specific immune cells uptake and influencing overall distribution after systemic administration.

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Article Pharmacology & Pharmacy

Efficient antiglioblastoma therapy in mice through doxorubicin-loaded nanomicelles modified using a novel brain-targeted RVG-15 peptide

Mingfeng Han et al.

Summary: The research team successfully synthesized a novel brain-targeted nanocarrier by reducing the size of the peptide derived from the rabies virus glycoprotein, and prepared DOX-loaded polymeric micelles with significant antiglioma effects.

JOURNAL OF DRUG TARGETING (2021)

Article Biology

Double Recombinant Vaccinia Virus: A Candidate Drug against Human Glioblastoma

Natalia Vasileva et al.

Summary: VV-GMCSF-Lact shows promising therapeutic potential for glioblastoma treatment by effectively reducing the viability of glioblastoma cells in vitro, crossing the blood-brain barrier, selectively replicating in orthotopically transplanted tumors, and inhibiting tumor xenograft and metastasis growth.

LIFE-BASEL (2021)

Article Virology

Intratumoral Virotherapy with Wild-Type Newcastle Disease Virus in Carcinoma Krebs-2 Cancer Model

Kseniya S. Yurchenko et al.

Summary: The study investigated the antitumor effect of oncolytic Newcastle disease virus (NDV) in treating mouse Krebs-2 carcinoma, demonstrating that intratumoral injections of NDV can inhibit tumor growth, induce necrosis-like changes in the tumor tissue, and have antiangiogenic effects.

VIRUSES-BASEL (2021)

Article Multidisciplinary Sciences

Viral infection of cells within the tumor microenvironment mediates antitumor immunotherapy via selective TBK1-IRF3 signaling

Michael C. Brown et al.

Summary: Recombinant poliovirus therapy primarily mediates antitumor immunotherapy via direct infection of non-malignant tumor microenvironment cells, independent of malignant cell lysis, by inducing selective innate inflammation and sustained type-I/III interferon release, leading to polyfunctional and Th1-differentiated antitumor T cell phenotypes.

NATURE COMMUNICATIONS (2021)

Article Oncology

Phase I trial of intratumoral PVSRIPO in patients with unresectable, treatment-refractory melanoma

Georgia M. Beasley et al.

Summary: In a phase I trial of intratumoral PVSRIPO in patients with unresectable melanoma, the treatment was well tolerated with promising antitumor activity, showing no serious adverse events reported and achieving an objective response rate of 33%, with some patients experiencing complete response in both injected and uninjected lesions.

JOURNAL FOR IMMUNOTHERAPY OF CANCER (2021)

Review Oncology

Repurposing Infectious Diseases Vaccines Against Cancer

Liese Vandeborne et al.

Summary: This review summarizes research activities using approved vaccines to treat cancer, with data supporting cancer therapeutic use for 16 vaccines. Clinical trials have been conducted or are ongoing for 10 vaccines, while preclinical evidence supports further evaluation of the remaining 6 vaccines.

FRONTIERS IN ONCOLOGY (2021)

Review Pharmacology & Pharmacy

Next Step in Gene Delivery: Modern Approaches and Further Perspectives of AAV Tropism Modification

Maxim A. Korneyenkov et al.

Summary: AAV is a popular choice for gene therapy delivery, but its unspecific tissue tropism is a serious limitation. Recent techniques have been developed to produce highly selective and targeted AAV capsids, although it is still far from the ideal state. Despite drawbacks and limitations, other approaches are still in play in the field of AAV research.

PHARMACEUTICS (2021)

Article Chemistry, Multidisciplinary

Long-Term Fate of Magnetic Particles in Mice: A Comprehensive Study

Ivan Zelepukin et al.

Summary: Safe application of nanoparticles in medicine requires a full understanding of their pharmacokinetics and degradation in the organism. In this study, a magnetic spectral approach was used for in vivo monitoring of magnetic particle degradation, revealing the significant influence of parameters such as dose, size, surface coating, and internal architecture on the degradation process. These findings provide deeper insights into particle degradation in vivo, potentially facilitating the rational design of nano- and microparticles with predictable long-term fate in vivo.

ACS NANO (2021)

Article Oncology

Phase 2 Trial of Oncolytic H-1 Parvovirus Therapy Shows Safety and Signs of Immune System Activation in Patients With Metastatic Pancreatic Ductal Adenocarcinoma

Jacek Hajda et al.

Summary: This study aimed to investigate the safety, clinical efficacy, pharmacokinetics, shedding, and immune response of an oncolytic parvovirus in patients with metastatic PDAC. Results showed excellent tolerability, partial response in some patients, and favorable immune activation after administration. The study met all primary objectives and can serve as a basis for further clinical development.

CLINICAL CANCER RESEARCH (2021)

Article Environmental Sciences

What we know and what we need to know about the origin of SARS-CoV-2

Jose L. Domingo

Summary: The origin of COVID-19 has been a topic of much debate, with some supporting a natural origin while others suggesting a possible unnatural origin. Clear evidence confirming the intermediate host of SARS-CoV-2 is still lacking, and investigations into the origin are crucial in preventing similar future pandemics.

ENVIRONMENTAL RESEARCH (2021)

Review Biochemistry & Molecular Biology

Step-by-Step Immune Activation for Suicide Gene Therapy Reinforcement

Irina Alekseenko et al.

Summary: Gene-directed enzyme prodrug gene therapy (GDEPT) is a promising method of chemotherapy for cancer with minimal side effects, but has not yet reached clinical application after over 25 years of research. Combination therapies incorporating immunomodulators alongside traditional methods like chemotherapy and radiotherapy, as well as GDEPT, may generate robust tumor-specific immune responses and show great potential in cancer treatment. Expanding research into immunotherapy and exploring the use of danger signals to enhance immune responses within tumors are important considerations for improving cancer therapy outcomes.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)

Article Oncology

Neural stem cell delivery of an oncolytic adenovirus in newly diagnosed malignant glioma: a first-in-human, phase 1, dose-escalation trial

Jawad Fares et al.

Summary: The study investigated the safety and activity of NSC-CRAd-S-pk7, an engineered oncolytic adenovirus delivered by neural stem cells (NSCs), in patients with newly diagnosed high-grade glioma. The treatment was found to be feasible and safe, supporting further investigation in a phase 2/3 clinical trial.

LANCET ONCOLOGY (2021)

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Efficacy and safety of oncolytic viruses in advanced or metastatic cancer: a network meta-analysis

Ruiyang Xie et al.

Summary: The study compared efficacy and safety of four oncolytic virus therapies, finding that T-VEC and T-VEC plus systemic treatment provided the best objective response rates but should be used with caution regarding grade >= 3 adverse events. Combining oncolytic virus therapies with immune-checkpoint inhibitors, rather than chemotherapy or target agents, tended to improve objective response rates without severe adverse events.

VIROLOGY JOURNAL (2021)

Review Virology

Virotherapy in Germany-Recent Activities in Virus Engineering, Preclinical Development, and Clinical Studies

Dirk M. Nettelbeck et al.

Summary: Virotherapy research in Germany focuses on developing and engineering oncolytic viruses for killing cancer cells directly and indirectly through immune responses and therapeutic protein delivery. Clinical trials explore the safety and efficacy of various virotherapeutics in combination therapies, aiming to implement effective treatments in oncology.

VIRUSES-BASEL (2021)

Review Virology

Combinatorial Approaches for Cancer Treatment Using Oncolytic Viruses: Projecting the Perspectives through Clinical Trials Outcomes

Alexander Malogolovkin et al.

Summary: Recent breakthroughs in cancer immunotherapy have transformed oncology and provided new hope for a cure. The combination of oncolytic viruses with immune checkpoint inhibitors shows promise, but finding the most effective combinations remains complex. This review highlights the efficacy, safety, and future perspectives of virotherapy based on clinical trial data.

VIRUSES-BASEL (2021)

Article Oncology

Polymer stealthing and mucin-1 retargeting for enhanced pharmacokinetics of an oncolytic vaccinia virus

Claudia Hill et al.

Summary: Vaccinia virus (VV) shows potential for cancer treatment due to its tumor tropism, but its clinical utility is limited by poor penetration into tumors. Chemical stealthing techniques improve the pharmacokinetics of VV. Experiments demonstrate that coating VV with amphiphilic polymer and attaching targeting antibodies can enhance its infectivity and circulation time in vivo, potentially improving its therapeutic effectiveness.

MOLECULAR THERAPY-ONCOLYTICS (2021)

Article Oncology

Efficient delivery of oncolytic enterovirus by carrier cell line NK-92

Elizaveta Sergeevna Podshivalova et al.

Summary: It has been found that natural killer cell line NK-92 can efficiently deliver cox-sackievirus A7 (CVA7) to tumors, potentially improving the treatment efficacy for glioblastoma multiforme and other forms of cancer.

MOLECULAR THERAPY-ONCOLYTICS (2021)

Review Oncology

Oncolytic Virus-Induced Autophagy in Glioblastoma

Margarita Kamynina et al.

Summary: Glioblastoma (GBM) is the most common and aggressive brain tumor with limited therapeutic options. Gene therapies using self-replicating oncolytic viruses may advance GBM treatment. Autophagy in glioblastoma plays a dual role in cell death and survival.

CANCERS (2021)

Article Multidisciplinary Sciences

A new insight into aggregation of oncolytic adenovirus Ad5-delta-24-RGD during CsCl gradient ultracentrifugation

Aleksei A. Stepanenko et al.

Summary: In this study, it was found that rAds with the RGD-4C peptide in the HI loop were more prone to aggregation during 2 x CsCl rather than iodixanol gradient ultracentrifugation. Additionally, substituting cysteine residues with free thiol groups with glycine prevented aggregation during 2 x CsCl gradient purification, but reduced cytolytic efficacy in certain cancer cell lines.

SCIENTIFIC REPORTS (2021)

Review Chemistry, Medicinal

Targeted Drug Delivery in Brain Tumors-nanochemistry Applications and Advances

Babak Ganjeifar et al.

Summary: Brain tumors remain a major health issue with limited treatment options due to systemic toxicity, blood-brain barrier limitations, and low selectivity of therapeutic agents for tumor cells. Advances in targeted drug delivery systems using nanoparticles show great potential in improving therapeutic outcomes for brain tumors. Ongoing research focuses on developing effective new anticancer agents and strategies for blood-brain barrier modulation and penetration.

CURRENT TOPICS IN MEDICINAL CHEMISTRY (2021)

Review Dermatology

Review of talimogene laherparepvec: A first-in-class oncolytic viral treatment of advanced melanoma

Karyn Haitz et al.

JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY (2020)

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Delivery and Biosafety of Oncolytic Virotherapy

Lizhi Li et al.

FRONTIERS IN ONCOLOGY (2020)

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Developing oncolytic viruses for clinical use: A consortium approach

Vera Kemp et al.

CYTOKINE & GROWTH FACTOR REVIEWS (2020)

Review Biochemistry & Molecular Biology

Oncolytic virotherapy for pancreatic ductal adenocarcinoma: A glimmer of hope after years of disappointment?

Evelyne Tassone et al.

CYTOKINE & GROWTH FACTOR REVIEWS (2020)

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Epidemics as an adaptive driving force determining lifespan setpoints

Peter V. Lidsky et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2020)

Review Biochemistry & Molecular Biology

Clinical Application of Oncolytic Viruses: A Systematic Review

Mary Cook et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020)

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Extracellular Vesicle-Based Therapeutics: Preclinical and Clinical Investigations

Natalia L. Klyachko et al.

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Recombinant Vaccinia Virus Promising for Melanoma Treatment

T. V. Bauer et al.

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Adaptation of the Newcastle Disease Virus to Cell Cultures for Enhancing Its Oncolytic Properties

K. S. Yurchenko et al.

ACTA NATURAE (2019)

Article Medicine, Research & Experimental

Oncolytic Activity of the Vaccine Strain of Type 3 Poliovirus on the Model of Rat Glioma C6 Cells

A. O. Sosnovtseva et al.

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE (2019)

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Engineering oncolytic vaccinia virus with functional peptides through mild and universal strategy

Li-Li Huang et al.

ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2019)

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Approaching infinite affinity through engineering of peptide-protein interaction

Anthony H. Keeble et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2019)

Article Biochemistry & Molecular Biology

Targeted Therapy of Human Glioblastoma Combining the Oncolytic Properties of Parvovirus H-1 and Attenuated Strains of the Vaccinia Virus

A. V. Tkacheva et al.

MOLECULAR GENETICS MICROBIOLOGY AND VIROLOGY (2019)

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Oncolytic Paramyxoviruses: Mechanism of Action, Preclinical and Clinical Studies

O. V. Matveeva et al.

MOLECULAR BIOLOGY (2018)

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The advent of oncolytic virotherapy in oncology: The Rigvir® story

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EUROPEAN JOURNAL OF PHARMACOLOGY (2018)

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Olga V. Matveeva et al.

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Recombinant Viruses for Cancer Therapy

Daria S. Chulpanova et al.

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A. S. Sidorenko et al.

BULLETIN OF RUSSIAN STATE MEDICAL UNIVERSITY (2018)

Article Medicine, General & Internal

CHANGES IN THE SENSITIVITY OF HUMAN GLIOBLASTOMA CELLS TO ONCOLYTIC ENTEROVIRUSES INDUCED BY PASSAGING

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BULLETIN OF RUSSIAN STATE MEDICAL UNIVERSITY (2018)

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Zika virus has an oncolytic activity against human glioblastoma U87 cells

V. A. Svyatchenko et al.

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HUMAN ENTEROVIRUSES EXHIBIT SELECTIVE ONCOLYTIC ACTIVITY IN THE MODEL OF HUMAN GLIOBLASTOMA MULTIFORME XENOGRAFTS IN IMMUNODEFICIENT MICE

A. O. Zheltukhin et al.

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Oncolytic Sendai Virus Therapy of Canine Mast Cell Tumors (A Pilot Study)

Galina V. Ilyinskaya et al.

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Zika virus has oncolytic activity against glioblastoma stem cells

Zhe Zhu et al.

JOURNAL OF EXPERIMENTAL MEDICINE (2017)

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Change in Oncolytic Activity of Sendai Virus during Adaptation to Cell Cultures

S. S. Zainutdinov et al.

MOLECULAR GENETICS MICROBIOLOGY AND VIROLOGY (2017)

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Highly Effective Production of Biologically Active, Secreted, Human Granulocyte-Macrophage Colony-Stimulating Factor by Recombinant Vaccinia Virus

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Sensitivity of C6 Glioma Cells Carrying the Human Poliovirus Receptor to Oncolytic Polioviruses

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BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE (2016)

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Oncolytic virus efficiency inhibited growth of tumour cells with multiple drug resistant phenotype in vivo and in vitro

Elena P. Goncharova et al.

JOURNAL OF TRANSLATIONAL MEDICINE (2016)

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Evolving lessons on nanomaterial-coated viral vectors for local and systemic gene therapy

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Features of the Antitumor Effect of Vaccinia Virus Lister Strain

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VIRUSES-BASEL (2016)

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Oncolysis by paramyxoviruses: multiple mechanisms contribute to therapeutic efficiency

Olga V. Matveeva et al.

MOLECULAR THERAPY-ONCOLYTICS (2015)

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Oncolysis by paramyxoviruses: preclinical and clinical studies

Olga V. Matveeva et al.

MOLECULAR THERAPY-ONCOLYTICS (2015)

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Mechanisms of Oncolysis by Paramyxovirus Sendai

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ONCOIMMUNOLOGY (2014)

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Oncolytic adenoviruses: A thorny path to glioma cure

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Apoptin enhances the oncolytic activity of vaccinia virus in vitro

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MOLECULAR BIOLOGY (2013)

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Targeted and Armed Oncolytic Poxviruses for Cancer: the Lead Example of JX-594

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Oncolytic enteroviruses

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MOLECULAR BIOLOGY (2012)

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Oncolytic adenoviruses in anticancer therapy: Current status and prospects

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MOLECULAR BIOLOGY (2012)

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Hyaluronidase Expression by an Oncolytic Adenovirus Enhances Its Intratumoral Spread and Suppresses Tumor Growth

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MOLECULAR THERAPY (2010)

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Mesenchymal stem cells effectively deliver an oncolytic adenovirus to intracranial glioma

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STEM CELLS (2008)

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Targeted inflammation during oncolytic virus therapy severely compromises tumor blood flow

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MOLECULAR THERAPY (2007)

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History of oncolytic viruses: Genesis to genetic engineering

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MOLECULAR THERAPY (2007)

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Intravascular adenoviral agents in cancer patients: Lessons from clinical trials

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SCIENCE (2002)

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Blood clearance rates of adenovirus type 5 in mice

R Alemany et al.

JOURNAL OF GENERAL VIROLOGY (2000)