Transient expression systems to rewire plant carotenoid metabolism

Enrichment of foodstuffs with health-promoting metabolites such as carotenoids is a powerful tool to fight against unhealthy eating habits. Dietary carotenoids are vitamin A precursors and reduce risk of several chronical diseases. Additionally, carotenoids and their cleavage products (apocarotenoids) are used as natural pigments and flavors by the agrofood industry. In the last few years, major advances have been made in our understanding of how plants make and store carotenoids in their natural compartments, the plastids. In part, this knowledge has been acquired by using transient expression systems, notably agroinfiltration and viral vectors. These techniques allow profound changes in the carotenoid profile of plant tissues at the desired developmental stage, hence preventing interference with normal plant growth and development. Here we review how transient expression approaches have contributed to learn about the structure and regulation of plant carotenoid biosynthesis and to rewire carotenoid metabolism and storage for efficient biofortification of plant tissues.

Automated summary

Enriching food with health-promoting compounds like carotenoids is an effective approach to combat unhealthy eating habits. Carotenoids are precursors of vitamin A and can reduce the risk of various chronic diseases. Recent advances in transient expression systems have provided valuable insights into the synthesis and storage of carotenoids in plants, enabling efficient biofortification of plant tissues.