期刊
CURRENT OPINION IN HEMATOLOGY
卷 29, 期 4, 页码 167-173出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/MOH.0000000000000714
关键词
clinical trial; ex-vivo expansion; hematopoietic stem and progenitor cells; preclinical study
类别
资金
- National Key Research and Development Project [2019YFA0111800]
- Major Research Plan of National Natural Science Foundation of China [91957107]
- General Program of National Natural Science Foundation of China [81970095]
- Recruitment Program of Global Experts of China
- Shanghai Eastern Scholar Program [TP2018047]
This review discusses the progress of ex-vivo expansion of human HSCs and HPCs, including the identification of a reliable marker for functional HSCs and the promotion of expansion by SR1 and UM171. Preliminary data from clinical trials demonstrate that ex-vivo expansion can overcome the rarity of HSCs without compromising stemness.
Purpose of review Ex-vivo expansion of hematopoietic stem cells (HSCs) is one potential approach to enhance the clinical efficacy of hematopoietic cell transplantation-based therapy for malignant and nonmalignant blood diseases. Here, we discuss the major progress of preclinical and clinical studies on the ex-vivo expansion of human HSCs and progenitor cells (HPCs). Recent findings Single-cell RNA sequencing identified ADGRG1 as a reliable marker of functional HSCs upon ex-vivo expansion-induced mitochondrial oxidative stress. Both SR1 and UM171 significantly promote ex-vivo expansion of human cord blood HSCs and HPCs, as determined in preclinical animal models. Encouraged by these findings from the bench, multiple phase I/II and phase II clinical trials have been conducted to evaluate the safety, feasibility and efficacy of SR1-expanded and UM171-expanded cord blood units in patients with hematological malignancy. Preliminary data from multiple phase I/II clinical trials regarding transplants of ex-vivo-expanded HSCs and HPCs have demonstrated that ex-vivo expansion may be used to overcome the limitation of the rarity of HSCs without compromising stemness.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据