Strategies for production of hydrophobic compounds

Article Agricultural Engineering

Metabolic engineering of Yarrowia lipolytica for improving squalene production

Wen-Yan Tang et al.

Summary: The study successfully increased the production of squalene in Yarrowia lipolytica using pathway engineering and bioprocess engineering, achieving significant experimental results.

BIORESOURCE TECHNOLOGY (2021)

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Article Biotechnology & Applied Microbiology

Production of β-carotene in Saccharomyces cerevisiae through altering yeast lipid metabolism

Yijin Zhao et al.

Summary: Regulating lipid metabolic pathways is crucial for increasing beta-carotene accumulation in Saccharomyces cerevisiae. Overexpression of sterol ester synthesis genes and deletion of phosphatidate phosphatase genes significantly enhance beta-carotene yield.

BIOTECHNOLOGY AND BIOENGINEERING (2021)

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Article Biotechnology & Applied Microbiology

Targeting pathway expression to subcellular organelles improves astaxanthin synthesis in Yarrowia lipolytica

Yongshuo Ma et al.

Summary: This study explores the strategy of expressing the astaxanthin biosynthesis pathway in sub-organelles of the oleaginous yeast Yarrowia lipolytica to achieve efficient synthesis of astaxanthin, leading to increased yield and reduced accumulation of intermediates. Targeting the astaxanthin pathway to subcellular organelles accelerates conversion and significantly improves synthesis, ultimately enhancing compartmentalized isoprenoid biosynthesis in Y. lipolytica.

METABOLIC ENGINEERING (2021)

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Article Biotechnology & Applied Microbiology

Metabolic compartmentalization in yeast mitochondria: Burden and solution for squalene overproduction

Zhan-Tao Zhu et al.

Summary: Harnessing mitochondria for terpene biosynthesis is promising but can lead to metabolic burden and poor cell growth. Analysis shows toxicity from MVA pathway phosphorylated intermediates within mitochondria inhibits cellular functions. Combining cytoplasmic and mitochondrial engineering strategies can alleviate metabolic burden and enhance terpene production.

METABOLIC ENGINEERING (2021)

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Article Chemistry, Multidisciplinary

Microbial Bioprocess for Extracellular Squalene Production and Formulation of Nanoemulsions

Jaehyun Park et al.

Summary: Engineering Corynebacterium glutamicum enabled the production and secretion of hydrophobic squalene without the need for in situ extractions, resulting in increased total squalene production. By promoting extraction and formulating bio-squalene nanoemulsions, creaming effects were analyzed for cosmetic applications. Combining metabolic pathway engineering and facilitated secretion in microbial cells shows promise for sustainable production of hydrophobic chemicals.

ACS SUSTAINABLE CHEMISTRY & ENGINEERING (2021)

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Article Biotechnology & Applied Microbiology

Rhodosporidium sp. DR37: a novel strain for production of squalene in optimized cultivation conditions

Shahryar Shakeri et al.

Summary: This study is the first to employ marine oleaginous Rhodosporidium sp. DR37 for accumulation of squalene in optimized medium. The findings suggest the potential of Rhodosporidium sp. DR37 for production of squalene, lipid, and carotenoids for biofuel applications on a large scale.

BIOTECHNOLOGY FOR BIOFUELS (2021)

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Article Biochemical Research Methods

Engineering the oleaginous yeast Yarrowia lipolytica for β-farnesene overproduction

Tianqiong Shi et al.

Summary: The study successfully engineered Yarrowia lipolytica to increase the production of beta-farnesene and further boosted the yield by knocking out acyltransferase genes. Additionally, fed-batch fermentation significantly enhanced the beta-farnesene titer, providing a new route for producing other terpenoids more efficiently.

BIOTECHNOLOGY JOURNAL (2021)

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Article Biochemical Research Methods

Highly Efficient Production of Menaquinone-7 from Glucose by Metabolically Engineered Escherichia coli

Quanxiu Gao et al.

Summary: This study improved the production of MK-7 in E. coli by optimizing the biosynthetic pathways and achieved high yields through combinational metabolic and membrane engineering strategies. The engineered E. coli strain developed in this study offers a promising and cost-effective alternative for MK-7 production from renewable feedstock.

ACS SYNTHETIC BIOLOGY (2021)

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Review Biochemical Research Methods

Efflux Transporters' Engineering and Their Application in Microbial Production of Heterologous Metabolites

Muhammad Saad Ahmed et al.

Summary: This study provides insights into efflux transport proteins as potential solutions for enhancing the export of heterologous metabolites from microbial cell systems, including their mechanistic modes of action, selection of appropriate transporters, and future research directions.

ACS SYNTHETIC BIOLOGY (2021)

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Article Biotechnology & Applied Microbiology

Increased Accumulation of Squalene in Engineered Yarrowia lipolytica through Deletion of PEX10 and URE2

Liu-Jing Wei et al.

Summary: This study demonstrated a novel metabolic design for enhancing squalene accumulation in Y. lipolytica by overexpressing mevalonate pathway enzymes, increasing lipid production, and deleting nitrogen catabolite repression-related genes. The results suggest that deletion of URE2 can be utilized to boost hydrophobic target product production in oleaginous yeast strains. This discovery holds potential for future engineering of cell factories to efficiently produce terpenoids.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2021)

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Article Biotechnology & Applied Microbiology

Engineering yeast subcellular compartments for increased production of the lipophilic natural products ginsenosides

Yusong Shi et al.

Summary: By targeting enzymes to specific subcellular compartments in yeast cells through synthetic biology, the efficiency of natural product biosynthesis can be significantly improved, providing a new strategy for engineering yeast to produce complex natural products.

METABOLIC ENGINEERING (2021)

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Article Biotechnology & Applied Microbiology

Metabolic engineering of Saccharomyces cerevisiae for production of beta-carotene from hydrophobic substrates

Zahra Fathi et al.

Summary: This study successfully engineered baker's yeast Saccharomyces cerevisiae to produce beta-carotene on hydrophobic substrates by expressing lipases and carotenogenic genes. The engineered strain reached a high titer of beta-carotene, demonstrating the potential of using lipases and hydrophobic substrate supplementation for carotenoid production in S. cerevisiae.

FEMS YEAST RESEARCH (2021)

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Article Chemistry, Applied