Bacterial hitchhikers derive benefits from fungal housing

Fungi and bacteria are ubiquitous constituents of all microbiomes, yet mechanisms of microbial persistence in polymicrobial communities remain obscure. Here, we examined the hypothesis that specialized fungal survival structures, chlamydospores, induced by bacterial lipopeptides serve as bacterial reservoirs. We find that symbiotic and pathogenic gram-negative bacteria from non-endosymbiotic taxa enter and propagate in chlamydospores. Internalized bacteria have higher fitness than planktonic bacteria when challenged with abiotic stress. Further, tri-cultures of Ralstonia solanacearum, Pseudomonas aeruginosa, and Aspergillus flavus reveal the unprecedented finding that chlamydospores are colonized by endofungal bacterial communities. Our work identifies a previously unknown ecological role of chlamydospores, provides an expanded view of microbial niches, and presents significant implications for the persistence of pathogenic and beneficial bacteria.

Automated summary

This study reveals that chlamydospores can serve as bacterial reservoirs, allowing symbiotic and pathogenic bacteria to survive and propagate in fungi, likely facilitated by bacterial lipopeptides. Furthermore, the colonization of endofungal bacterial communities in chlamydospores suggests a previously unknown ecological role. These findings provide a broader understanding of microbial niches and have significant implications for pathogenic and beneficial bacteria persistence.