4.8 Article

Isolation, profiling, and tracking of extracellular vesicle cargo in Caenorhabditis elegans

期刊

CURRENT BIOLOGY
卷 32, 期 9, 页码 1924-+

出版社

CELL PRESS
DOI: 10.1016/j.cub.2022.03.005

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资金

  1. National Institutes of Health (NIH) [DK116606, DK059418, NS120745]
  2. National Science Foundation (NSF) [DBI 1936046]
  3. NIH [S10OD025140, S10OD016400, R35 GM124976, K12 GM093854]
  4. HGINJ
  5. Donald Stahlin Memorial Fund
  6. NIH Office of Research Infrastructure Programs [P40 OD010440]
  7. [R24OD010943]

向作者/读者索取更多资源

Extracellular vesicles (EVs) can mediate intercellular communication by carrying protein and RNA cargo. C. elegans is a tractable animal system for studying and identifying EV cargo. The study found that EVs in C. elegans also carry RNA, suggesting a potential role for EVs in extracellular RNA-based communication.
Extracellular vesicles (EVs) may mediate intercellular communication by carrying protein and RNA cargo. The composition, biology, and roles of EVs in physiology and pathology have been primarily studied in the context of biofluids and in cultured mammalian cells, The experimental tractability of C. elegans makes for a powerful in vivo animal system to identify and study EV cargo from its cellular source. We developed an innovative method to label, track, and profile EVs using genetically encoded, fluorescent-tagged EV cargo and conducted a large-scale isolation and proteomic profiling. Nucleic acid binding proteins (similar to 200) are overrepresented in our dataset. By integrating our EV proteomic dataset with single-cell transcriptomic data, we identified and validated ciliary EV cargo: CD9-like tetraspanin (TSP-6), ectonucleotide pyrophosphatase/phosphodiesterase (ENPP-1), mini-chromosome maintenance protein (MCM-3), and double-stranded RNA transporter SID-2. C. elegans EVs also harbor RNA, suggesting that EVs may play a role in extracellular RNA-based communication.

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