期刊
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
卷 21, 期 3, 页码 2688-2714出版社
WILEY
DOI: 10.1111/1541-4337.12965
关键词
allosteric effects; enzymatic catalysis; food industry; lipase lid; rebuilding approaches
资金
- National Natural Science Foundation of China [32130084, 31972029]
- National Key R&D Program of China [2018YFC1602101]
- Ten Thousand Talent Program Youth Top-notch Talent Project of China
- Youth Fund of Zhejiang Province [LQ22C200004]
This review presents the potential applications of lipases in esterification, amidation, and transesterification, emphasizing the importance of the lid in lipase catalysis. The opportunities and advantages of rebuilding the lid region using biotechnologies and enzyme immobilization techniques are discussed, along with their limitations and proposed solutions. The review also highlights the future prospects of designing hyperfunctional lipases for the food industry based on lid conformation and dynamics.
The applications of lipases in esterification, amidation, and transesterification have broadened their potential in the production of fine compounds with high cumulative values. Mostly, the catalytic triad of lipases is covered by either one or two mobile peptides called the lid that control the substrate channel to the catalytic center. The lid holds unique conformational allostery via interfacial activation to regulate the dynamics and catalytic functions of lipases, thereby highlighting its importance in redesigning these enzymes for industrial applications. The structural characteristic of lipase, the dynamics of lids, and the roles of lid in lipase catalysis were summarized, providing opportunities for rebuilding lid region by biotechniques (e.g., metagenomic technology and protein engineering) and enzyme immobilization. The review focused on the advantages and disadvantages of strategies rebuilding the lid region. The main shortcomings of biotechnologies on lid rebuilding were discussed such as negative effects on lipase (e.g., a decrease of activity). Additionally, the main shortcomings (e.g., enzyme desorption at high temperatre) in immobilization on hydrophobic supports via interfacial action were presented. Solutions to the mentioned problems were proposed by combinations of computational design with biotechnologies, and improvements of lipase immobilization (e.g., immobilization protocols and support design). Finally, the review provides future perspectives about designing hyperfunctional lipases as biocatalysts in the food industry based on lid conformation and dynamics.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据