期刊
COMPARATIVE MEDICINE
卷 72, 期 2, 页码 78-89出版社
AMER ASSOC LABORATORY ANIMAL SCIENCE
DOI: 10.30802/AALAS-CM-21-000082
关键词
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资金
- American College of Laboratory Animal Medicine (ACLAM) Foundation
- Colorado University Denver GI and Liver Innate Immunity Program
This study evaluated the effectiveness of oral prophylactic antibiotics in preventing C. bovis infection and analyzed their impact on the skin microbiome of mice. The results showed that 14 days of prophylactic antibiotics can prevent the persistence of C. bovis infection. However, the effects of antibiotics on commensal bacteria and potential impacts on xenograft models need to be further considered.
Corynebacterium bovis is an opportunistic pathogen of the skin of immunodeficient mice and is sensitive to oral antibiotics that reach therapeutic blood concentrations. However, prophylactic antibiotics are considered to be ineffective at preventing C. bovis infection. In addition, the effect of C. bovis on the skin microbiome (SM) of common immunodeficient mouse strains has yet to be characterized. Consequently, we evaluated whether oral prophylactic antibiotics prevent C. bovis infection after inoculation. An infectious dose of C. bovis was applied to the skin of Hsd:Athymic Nude (nude) and NOD.Cg-Prkdc(scid) I/2rg(tm1wjl)/SzJ (NSG) mice. Mice were then housed individually and assigned randomly to receive either untreated drinking water (Cb+Abx- group) or prophylactic amoxicillin-clavulanic acid in the drinking water (0.375 mg/mL) for 14 d (Cb+Abx+ group). A third treatment group of each mouse strain was uninoculated and untreated (Cb-Abx-group). Mice from all groups were serially sampled by using dermal swabs to monitor C. bovis infection via quantitative real-time PCR and the SM via 16S rRNA sequence analysis. Fourteen days of prophylactic antibiotics prevented the perpetuation of C. bovis skin infection in both strains. Only the combination of C. bovis inoculation and oral antibiotics (Cb+Abx+) significantly affected the SM of NSG mice at day 14; this effect resolved by the end of the study (day 70). In mice that did not receive antibiotics, C. bovis significantly altered the SM of nude mice but not NSG mice at days 14 and 70. These findings demonstrate the potential benefit of prophylactic antibiotics for prevention of C. bovis infection. However, indirect effect of antibiotics on commensal bacteria and potential effects on xenograft models must be considered.
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