Mesoporous bioactive glass/bacterial cellulose composite scaffolds for bone support materials

In this study, a nanocomposite scaffold composed of mesoporous bioactive glass/bacterial cellulose (MBG/BC) has been synthesized using an in situ membrane-liquid interface culture. In the resulting MBG/BC nano composites, MBG is homogeneously distributed in the three-dimensional BC matrix. In vitro mineralization experiments revealed that incorporating MBG into BC scaffolds enhanced their bioactivity. Subsequently, human bone marrow stromal cells (hMBSCs) were seeded onto MBG/BC scaffolds, and the effect of MBG on biocompatibility and bone regeneration was evaluated by analyzing the cell morphology, measuring cell viability, alkaline phosphatase (ALP) activity, and osteogenic-related gene expression. The results showed that MBG/BC nanocomposite scaffolds exhibited an increase in hMBSC proliferation, ALP activity, and osteogenic-related gene expression compared to bare BC scaffolds. We believe that the scalable and simple green method can be used in fabricating other BC-based nanocomposite scaffolds, and that the MBG/BC scaffolds are considered a promising candidate for treatment of bone defects.

Automated summary

In this study, a nanocomposite scaffold composed of mesoporous bioactive glass/bacterial cellulose (MBG/BC) was synthesized using an in situ membrane-liquid interface culture. The incorporation of MBG into BC scaffolds enhanced their bioactivity, as demonstrated by in vitro mineralization experiments. Furthermore, the MBG/BC nanocomposite scaffolds promoted human bone marrow stromal cell proliferation, alkaline phosphatase activity, and osteogenic-related gene expression, indicating their potential for bone defect treatment.