Compound distribution, structural analysis and nanomechanical properties of nanofibers loaded with high-oleic palm oil nanoemulsions for packaging application

The effect of the HOPO concentration on the morphology, fiber diameter, oil load and distribution, protein structure, protein-oil colocalization, and nanomechanical properties of the NFs were evaluated. We obtained smooth and defect-free NFs with diameters in range of 77-94 nm. Fourier-transform infrared spectroscopy was used as a reliable method to confirm oil load and protein structure after electrospinning. beta-turn, beta-sheet, alpha-helix, and triple helical structures were quantified. Confocal laser scanning microscopy was used to analyze colocalization, and results revealed a high distribution of NE droplets along the NFs and high oil-protein colocalization (overlap coefficient of similar to 0.63). The nanomechanical properties of the NE-loaded NFs demonstrated that at higher HOPO concentrations, the elastic modulus decreased. Likewise, homogeneity and synergy between compounds were observed. The adhesion properties of NE-loaded NFs were associated with their sticky surface, which was attributed to presence of HOPO. The NE-loaded NFs presented suitable physical properties for edible packaging.

Automated summary

This study evaluated the effect of HOPO concentration on various properties of NFs and found that higher HOPO concentration decreased the elastic modulus of NFs and increased their adhesive properties, making them suitable for use as edible packaging.