4.2 Article

A cryo-fixation protocol to study the structure of the synaptonemal complex

期刊

CHROMOSOME RESEARCH
卷 30, 期 4, 页码 385-400

出版社

SPRINGER
DOI: 10.1007/s10577-022-09689-2

关键词

Synaptonemal complex; Meiosis; Cryo-fixation; Close-to-native structure

资金

  1. Swedish Research Council [2017-01853]
  2. Hospital Infantil de Mexico Federico Gomez [HIM/2018/079 SSA 1518, HIM/2021/061]
  3. Swedish Research Council [2017-01853] Funding Source: Swedish Research Council

向作者/读者索取更多资源

This study analyzed the structure of the synaptonemal complex (SC) using cryo-fixation and electron microscopy. Compared to chemical fixation, cryo-fixation showed differences in the width of the central region of the SC and the density of the transverse filaments.
Genetic variability in sexually reproducing organisms results from an exchange of genetic material between homologous chromosomes. The genetic exchange mechanism is dependent on the synaptonemal complex (SC), a protein structure localized between the homologous chromosomes. The current structural models of the mammalian SC are based on electron microscopy, superresolution, and expansion microscopy studies using chemical fixatives and sample dehydration of gonads, which are methodologies known to produce structural artifacts. To further analyze the structure of the SC, without chemical fixation, we have adapted a cryo-fixation method for electron microscopy where pachytene cells are isolated from mouse testis by FACS, followed by cryo-fixation, cryo-substitution, and electron tomography. In parallel, we performed conventional chemical fixation and electron tomography on mouse seminiferous tubules to compare the SC structure obtained with the two fixation methods. We found several differences in the structure and organization of the SC in cryo-fixed samples when compared to chemically preserved samples. We found the central region of the SC to be wider and the transverse filaments to be more densely packed in the central region of the SC.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.2
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据