4.7 Article

A novel TICT-based near-infrared fluorescent probe for light-up sensing and imaging of human serum albumin in real samples

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CHINESE CHEMICAL LETTERS
卷 34, 期 2, 页码 -

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ELSEVIER SCIENCE INC
DOI: 10.1016/j.cclet.2022.05.071

关键词

Human serum albumin; Near-infrared probe; High-throughput detection; Confocal imaging

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Human serum albumin (HSA) serves as an important biomarker and prognostic indicator for various human diseases. A novel near-infrared fluorescent probe (7-HTCF) was developed for real-time sensing and in-situ imaging of HSA. The probe exhibited excellent selectivity, good chemical stability, rapid fluorescent response, and ultra-high sensitivity for HSA detection. It can be used for quantitative detection of HSA in diluted plasma samples and for in-situ imaging of reabsorbed HSA in living cells.
Human serum albumin (HSA) has emerged as a pivotal biomarker and prognostic indicator for various human diseases. Real-time sensing and visual tracking of HSA in plasma or other biological systems will immensely facilitate the basic researchers and clinicians to better understand HSA-associated biological processes. Herein, a novel near-infrared (NIR) fluorescent probe (7-HTCF) was rationally constructed for light-up sensing and in-situ imaging of HSA in real samples, based on the principle of twisted intramolecular charge transfer (TICT). Under physiological conditions, 7-HTCF could be efficiently trapped by HSA to form a stable complex via binding on a non-drug binding site, while the complex emitted strong fluoresce signals around 670 nm. Further investigations demonstrated that 7-HTCF displayed a great combination of excellent selectivity and good chemical stability, as well as rapid fluorescent response and ultra-high sensitivity for HSA detection. Particularly, the newly developed light-up probe has been successfully utilized for quantitative detection of HSA in diluted plasma samples, while its readouts are hardly affected by the addition of therapeutic agents and herbal medicines. 7-HTCF is also successfully used for in-situ imaging of the reabsorbed HSA in living renal cells, while this dye exhibits good cell permeability and high resolution for in-situ imaging in living cells. Collectively, a novel TICT-based near-infrared fluorescent probe was devised for highly selective and ultra-sensitive sensing of HSA in plasma samples or imaging HSA in living cells, which offered a practical tool for clinical tests and for exploring HSA-associated biological processes.(c) 2022 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences.

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