期刊
CHINESE CHEMICAL LETTERS
卷 33, 期 9, 页码 4223-4228出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cclet.2022.03.008
关键词
Bioorthogonal reaction; Suzuki-Miyaura cross-coupling; Fluorogenic probes; Naphthalimide; Live-cell imaging
资金
- Beijing Nova Program [Z20110 0 0 06820049]
- National Natural Science Foundation of China [21907109]
- CAMS Innovation Fund for Graduate Students [2019-1007-03]
This study presents a general strategy for the in situ assembly of intramolecular charge-transfer (ICT)-based light up fluorophores via bioorthogonal Suzuki-Miyaura cross-coupling reaction. The designed fluorophores showed significant fluorescence enhancement and high fluorescence quantum yield after reaction with aryl boronic acid or boronate. They have been successfully applied in peptide conjugation, no-wash fluorogenic protein labeling, and mitochondria-selective bioorthogonal imaging in live cells.
Herein we presented a general strategy for in situ assembly of intramolecular charge-transfer (ICT)-based light up fluorophores via bioorthogonal Suzuki-Miyaura cross-coupling reaction. By introducing iodo group at the appropriate position, five fluorophores with different scaffolds including naphthalimide, coumarin, naphthalene sulfonate, nitrobenzoxadiazole, and acetonaphthone, were designed as bioorthogonal multicolor fluorogenic probes, which could produce significant fluorescence enhancement and high fluorescence quantum yield after Suzuki-Miyaura reaction with aryl boronic acid or boronate. Manipulating the substituents and pi scaffold in the fluorophores allows fine-tuning of their photophysical properties. With this strategy, we succeeded in peptide conjugation, no-wash fluorogenic protein labeling, and mitochondria-selective bioorthogonal imaging in live cells. (C) 2022 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences.
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