Response of rotating biological contactor started up by heterotrophic nitrification-aerobic denitrification bacteria to various C/N ratios

To improve the low pollutant removal efficiency of traditional biological methods for treating livestock and poultry breeding wastewater under a relatively low temperature, a rotating biological contactor (RBC) inoculated with heterotrophic nitrification-aerobic denitrification (HN-AD) bacteria was designed. A quick start-up process and a well removal performance had been achieved in the novel RBC. To elucidate the anti-load shock ability of the novel RBC, the effects of C/N ratio on nitrogen removal and microbial assemblage were focused under a low temperature (12 +/- 2 degrees C). Results showed that the highest NH4+-N and TN removal efficiency were 99.57 +/- 0.31% and 68.41 +/- 0.52%. Microbial diversity analysis based on high throughput sequencing technique showed that Arcobacter and Flavobacterium with an increasing relative abundance were the key to ensure high nitrogen removal efficiently at a low C/N ratio and temperature. Moreover, nitrogen transferring pathways of the novel RBC was revealed and dissimilatory nitrate reduction and denitrification were the main pathways. The excellent pollutant removal performance demonstrates that the novel RBC is a promising process to effectively treat wastewater with low C/N ratio and low temperature.

Automated summary

This study designed a rotating biological contactor (RBC) and inoculated it with heterotrophic nitrification-aerobic denitrification (HN-AD) bacteria to improve the low pollutant removal efficiency of traditional biological methods for treating livestock and poultry breeding wastewater under a relatively low temperature. The results showed that the novel RBC achieved high nitrogen removal efficiency and revealed the key microbial assemblage and nitrogen transferring pathways under low C/N ratio and temperature.