期刊
CHEMOSPHERE
卷 297, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.chemosphere.2022.134234
关键词
PFOS; ROS; Neurobehavioral; Dopaminergic system
资金
- National Natural Science Foundation of China [82173471, 82003409, 82073503, 81950410633, 42107467]
- Natural Science Foundation of Guang-dong Province [2021A1515012212, 2021A151011754, 2021B1515020015, 2020A1515011131, 2019A050510017, 2018B05052007, 2017A090905042]
- Science and Tech-nology Program of Guangzhou City [201807010032, 201803010054, 201903010023, 202102021014]
- Basic Research Foundation of the National Commonwealth Research Institute [PM-zx703-202104-057]
This study found that exposure to perfluorooctane sulfonates (PFOS) can cause neurobehavioral abnormalities in zebrafish larvae, affecting their neurodevelopment. This may be related to the increased reactive oxygen species (ROS) levels in the heads of zebrafish larvae and the disrupted dopamine signaling pathway. In addition, PFOS also induces developmental damage, such as increased malformation rate and shorter body length.
It has been reported that exposure to perfluorooctane sulfonates (PFOS) causes behavioral abnormalities in zebrafish larvae, but the possible mechanisms underlying these changes remain unexplored. In this study, zebrafish embryos (2 h postfertilization, 2-hpf) were exposed to PFOS at different concentrations (0, 0.032, 0.32 and 3.2 mg/L) for 120 h. Developmental endpoints and the locomotion behavior of larvae were evaluated. Reactive oxygen species (ROS) levels, dopamine contents, several genes and proteins related to neurodevelopment and dopamine signaling were examined. Our results indicate that increased ROS levels in the zebrafish larvae heads may be causally associated with neurodevelopment damage. Meanwhile, brain-derived neurotrophic factor (BDNF) and alpha1-Tubulin (alpha 1-Tubulin) protein contents were significantly increased, which may be a compensatory mechanism for the impaired central nervous system. PFOS-induced locomotor hyperactivity was observed in the first light phase and dark phase at the 0.32 and 3.2 mg/L of PFOS. Upregulation of dopamine-related genes tyrosine hydroxylase (th) and dopamine transporter (dat) associated with increased dopamine contents in the 3.2 mg/L of PFOS. In addition, protein expression of TH and DAT were noted at the 0.32 and 3.2 mg/L of PFOS concentrations. Our results suggested that PFOS induces neurobehavioral changes in zebrafish larvae, possibly by perturbing a dopamine signaling pathway. In addition, PFOS induced development damage, such as increased malformation rate and shorter body length.
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