期刊
CHEMOSPHERE
卷 291, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.chemosphere.2021.132995
关键词
Reduced graphene oxide; SARS-CoV-2 spike pseudovirus; Human enteric viruses; Virion adsorption
资金
- National Key R&D Program of China [2017YFC1601204, 2018YFC1602500]
- Tianjin Municipal Natural Science Foundation [19JCZDJC39900]
The study demonstrates that CTAB-functionalized rGO-Fe3O4 composites have efficient adsorption capabilities for human enteric viruses and SARS-CoV-2, and can be used in complex water environments. Additionally, the composite can help concentrate virus particles before qPCR analysis, significantly improving detection sensitivity.
The latent dangers of waterborne viral transmission have become a major public health concern. In this study, reduced graphene oxide (rGO)-Fe3O4 nanoparticles were decorated with cetyltrimethylammonium bromide (CTAB) to adsorb severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike pseudovirus and three human enteric viruses (HuNoV, HRV, and HAdV). The successful combination of CTAB with rGO-Fe3O4 was confirmed by transmission electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, zeta potential, Brunner-Emmet-Teller, and vibrating sample magnetometer measurements. The adsorption of HuNoV and HAdV followed pseudo-first-order kinetics, while that of HRV conformed to the pseudo-second-order model. CTAB-functionalized rGO-Fe3O4 exhibited exceptionally high adsorption of HuNoV, HRV, HAdV and SARS-CoV2 spike pseudovirus, with maximum adsorption capacities of 3.55 x 10(7), 7.01 x 10(7), 2.21 x 10(7) and 6.92 x 10(6) genome copies mg(- 1), respectively. Moreover, the composite could effectively adsorb the four types of virus particles from coastal, tap, and river water. In addition, concentrating the virions using CTAB functionalized rGO-Fe3O4 composites before qPCR analysis significantly improved the detection limit. The results indicate that viruses are captured on the surface of CTAB functionalized rGO-Fe3O4 composites through electrostatic interactions and the intrinsic adsorption ability of rGO. Overall, CTAB-functionalized rGO-Fe3O4 composites are promising materials for the adsorption and detection of human enteric viruses as well as SARS-CoV-2 from complex aqueous environments.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据