期刊
CHEMICAL ENGINEERING JOURNAL
卷 436, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.cej.2022.135205
关键词
SiC-CS; Catalase-like activity; Cervical cancer; Multi-signal readout
资金
- NationalNatural Science Foundation of China [21877012, 21575024]
- National Science Foundation of Fujian Province [2020 J02034, 2019 J01052063]
- Health-Education Joint Research Project of Fujian Province [2019-WJ-04]
In this study, a photothermal triggered multi-signal point-of-care testing protocol was developed for the determination of Human papillomavirus type 16 E6 protein in clinical vagina swabs. The protocol integrated a portable vagina swab as a sensing substrate, a composite probe SiC-CS@Ag as a label, and a flexible fluorescence-temperature indicator (FLTI) with thermal-responsive fluorescence capability. The system achieved sensitive target detection and provided rapid and precise methodologies for biomarker detection, presenting potential for early diagnosis of malignant diseases in remote areas.
In this protocol, a photothermal triggered multi-signal readout point-of-care testing (POCT) was established in a multifunctional clinical vagina swab for Human papillomavirus type 16 E6 (E6 protein) determination. Particularly, a portable vagina swab was employed as a sensing substrate integrated collection and analysis of samples. The composite probe SiC-CS@Ag with the chitosan (CS) decorated silicon carbide nanoparticles (SiC NPs) as a label was bounded to the swab via competitive reaction. To transfer recognition events into detectable signals, a flexible fluorescence-temperature indicator (FLTI) with thermal-responsive fluorescence capability was assembled on swab surface followed by sealing it into a home-made tiny pressure device and H2O2 & nbsp;as a signal reporter was introduced. Under near-infrared (NIR) laser expose, remarkable photothermal effect from SiC-CS@Ag caused notable temperature elevation, which lead to significant fluorescence (FL) quenching on FLTI. Meanwhile, increased temperature also enhance the catalase-like activity of SiC-CS@Ag toward H2O2 & nbsp;decomposition that cause significant pressure elevation. This bioassay realized sensitive target detection in the linear ranger from 10(-6) ng/mL to 1 ng/mL with detection limits of 1.60 x 10(-6) ng/mL. Such a photothermal mediated multi-signal readout sensing platform not only provides rapid and precise methodologies for biomarker detection, but also enriches strategies for POCT and boosted its application for early diagnosis of malignant diseases in remote areas.
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