期刊
CHEMICAL ENGINEERING JOURNAL
卷 437, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.cej.2022.135267
关键词
Biomimetic cascade nanoreactor; ZIF-67 nanocubes; Signal amplification; Colorimetric biosensing; Acid phosphatase
资金
- National Natural Science Foundation of China [21974053]
- Natural Science Foundation of Shandong Province [ZR2020QF094]
- Qingdao Major Scientific and Technological Project for Distinguished Scholars [20170103]
- Youth Innovation Team Talent Introduction Program ofShandong Province [20190164]
In this study, a new type of biomimetic cascade nanoreactor was designed and utilized for acid phosphatase (ACP) detection. The nanoreactor exhibited triple-enzyme mimetic activities and achieved ACP colorimetric detection through a cascade amplification strategy. This Co,N-CNC nanoreactor displayed promising applications in biosensing, medical diagnostics, and biotechnological research.
Delighted by the high catalytic efficiency and superior selectivity of multiple enzyme-driven biological catalytic cascades, biomimetic cascade nanoreactors have drawn much attention recently. Here, a new type of biomimetic cascade nanoreactor - Co nanoparticles embedded N-enriched carbon nanocubes (Co,N-CNC) which derived from ZIF-67 nanocubes was designed and utilized for acid phosphatase (ACP) detection. The distinctive composition and cubic structure endowed Co,N-CNC with significantly enhanced triple-enzyme mimetic activities, including oxidase (OXD)-, peroxidase (POD)-, and catalase (CAT)-like activities. Employing the OXD-and POD-like activities of Co,N-CNC nanoreactor, a cascade amplification strategy was proposed for the first time for ACP colorimetric detection without the assistance of destructive H2O2. Based on the high catalytic activity of Co, N-CNC and the cascade amplification strategy, a good linear relationship was obtained from 0.01 to 10.0 mU/mL, with a lower detection limit of 6.1 mu U/mL. Significantly, the Co,N-CNC could detect ACP in human serum samples with high accuracy. Therefore, the Co,N-CNC nanoreactor with triple-enzyme mimetic activities displayed broad prospects for biosensing, medical diagnostics and biotechnological research.
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