期刊
CHEMICAL ENGINEERING JOURNAL
卷 433, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.cej.2021.133624
关键词
Sulfur quantum dots; MOF-5-NH2; Self-cycling catalyzed hairpin assembly; Fluorescent aptasensor; Patulin; Detection
资金
- national key R&D projects of the 13th five year plan of China [2019YFC1606703]
Researchers have developed a simple, effective, and sensitive fluorescent aptasensor for the detection of low molecular weight mycotoxin patulin in apple juice. The aptasensor utilizes sulfur quantum dots encapsulated in MOF-5-NH2 as a fluorescent probe and self-cycling catalyzed hairpin assembly for signal amplification. The aptasensor demonstrates high sensitivity and satisfactory specificity, anti-interference, and reproducibility.
Tools to detect low molecular weight mycotoxin patulin (PAT) are urgently needed because of its harm to human health. Here, we report a simple, effective, and sensitive fluorescent aptasensor based on sulfur quantum dots encapsulated in MOF-5-NH2 (SQDs@MOF-5-NH2) and self-cycling catalyzed hairpin assembly (scCHA) for the detection of PAT in apple juice. The SQDs@MOF-5-NH2 synthesized by a bottle-around-ship solvothermal method works as a fluorescent probe and offers enhanced fluorescence intensity and good water-solubility versus MOF-5-NH2 due to the encapsulation of water-soluble SQDs. Meanwhile, the scCHA designed by the two DNA hairpins is initiated after the formation of PAT/aptamer, which further recycles PAT to effectively capture multiple fluorescence probes (SQDs@MOF-5-NH2) and amplify the detection signal. Attributed to the synergistic effect between the strong fluorescence intensity of SQDs@MOF-5-NH2 and the efficient PAT utilization of scCHA, the fluorescent aptasensor exhibits high sensitivity with detection limits as low as 0.753 pg/mL. The system has satisfactory specificity, anti-interference, and reproducibility (RSDs of intra-assay and inter-assay were 3.24% and 8.60%, respectively). The recoveries in apple juice were between 89.03% and 107.67%, which were with good consistency versus high-performance liquid chromatography (HPLC). These data collectively show that the developed fluorescent aptasensor has a good practical application value.
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