4.4 Article

Discovery of Novel Tyrosine Ammonia Lyases for the Enzymatic Synthesis of p-Coumaric Acid

期刊

CHEMBIOCHEM
卷 23, 期 10, 页码 -

出版社

WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.202200062

关键词

biocatalysis; flavonoids; p-coumaric acid; phenylalanine ammonia lyase; tyrosine ammonia lyase

资金

  1. European Union's Horizon 2020 Research and Innovation Programme [814650]
  2. Projekt DEAL

向作者/读者索取更多资源

p-Coumaric acid (p-CA) is a key precursor for flavonoid biosynthesis, and tyrosine ammonia lyases (TALs) catalyze the synthesis of p-CA from l-tyrosine. In this study, 875 putative TALs and 46 putative phenylalanine/tyrosine ammonia lyases (PTALs) were analyzed, and 5 TALs and 3 PTALs were characterized and found to exhibit the proposed enzymatic activity. The TAL from Chryseobacterium luteum sp. nov (TAL(clu)) showed the highest affinity and conversion efficiency towards l-tyrosine. Optimization of reaction conditions resulted in p-CA yields of 2.03 g/L after 8 hours by TAL(clu) and 2.35 g/L after 24 hours by TAL from Rivularia sp. PCC 7116 (TAL(rpc)). These TALs are potential candidates for the production of flavonoid precursor p-CA using whole-cell systems.
p-Coumaric acid (p-CA) is a key precursor for the biosynthesis of flavonoids. Tyrosine ammonia lyases (TALs) specifically catalyze the synthesis of p-CA from l-tyrosine, which is a convenient enzymatic pathway. To explore novel and highly active TALs, a phylogenetic tree-building approach was conducted including 875 putative TALs and 46 putative phenylalanine/tyrosine ammonia lyases (PTALs). Among them, 5 TALs and 3 PTALs were successfully characterized and found to exhibit the proposed enzymatic activity. The TAL from Chryseobacterium luteum sp. nov (TAL(clu)) has the highest affinity (K-m=0.019 mm) and conversion efficiency (k(cat)/K(m=)1631 s(-1) . mm(-1)) towards l-tyrosine. The reaction conditions for two purified enzymes and their E. coli recombinant cells were optimized and p-CA yields of 2.03 g/L after 8 hours by TAL(clu) and 2.35 g/L after 24 h by TAL from Rivularia sp. PCC 7116 (TAL(rpc)) in whole cells were achieved. These TALs are thus candidates for the construction of whole-cell systems to produce the flavonoid precursor p-CA.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.4
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据