期刊
CELLULAR AND MOLECULAR LIFE SCIENCES
卷 79, 期 4, 页码 -出版社
SPRINGER BASEL AG
DOI: 10.1007/s00018-022-04229-x
关键词
RNase; Virus; RSV; miRNA; tRNA; tRF
资金
- Universitat Autonoma de Barcelona
- Ministerio de Economia y Competitividad [SAF201566007P]
- FEDER funds
- Agencia Estatal de Investigacion [PID2019-106123GB-I00/AEI]
- Fundacio La Marato de TV3 [2080310]
- China Scholarship Council (CSC)
RNase2, the most abundant member of the RNaseA family in macrophages, has been found to have antiviral effects. In this study, RNase2 was knocked out in THP-1 cells and the response to Respiratory Syncytial Virus (RSV) was analyzed. RSV was found to induce expression of RNase2, which significantly enhanced cell survival. Sequencing analysis revealed that tRNA-derived fragments (tRFs) and second miRNAs were mostly accumulated in WT cells compared to KO cells.
RNase2 is the member of the RNaseA family most abundant in macrophages. Here, we knocked out RNase2 in THP-1 cells and analysed the response to Respiratory Syncytial Virus (RSV). RSV induced RNase2 expression, which significantly enhanced cell survival. Next, by cP-RNAseq sequencing, which amplifies the cyclic-phosphate endonuclease products, we analysed the ncRNA population. Among the ncRNAs accumulated in WT vs KO cells, we found mostly tRNA-derived fragments (tRFs) and second miRNAs. Differential sequence coverage identified tRFs from only few parental tRNAs, revealing a predominant cleavage at anticodon and D-loops at U/C (B1) and A (B2) sites. Selective tRNA cleavage was confirmed in vitro using the recombinant protein. Likewise, only few miRNAs were significantly more abundant in WT vs RNase2-KO cells. Complementarily, by screening of a tRF & tiRNA array, we identified an enriched population associated to RNase2 expression and RSV exposure. The results confirm the protein antiviral action and provide the first evidence of its cleavage selectivity on ncRNAs. [GRAPHICS] .
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