4.4 Article

Expression of the relaxin family peptide 4 receptor by enterochromaffin cells of the mouse large intestine

期刊

CELL AND TISSUE RESEARCH
卷 389, 期 1, 页码 1-9

出版社

SPRINGER
DOI: 10.1007/s00441-022-03635-8

关键词

INSL5; 5-HT; Enteroendocrine cells; Enteric nervous system; Colonic reflexes

资金

  1. NHMRC [APP1145686, APP1182996]
  2. MRC-UK [MRC_MC_UU_12012/3]
  3. Wellcome Trust [220271/Z/20/Z]
  4. Wellcome Trust [220271/Z/20/Z] Funding Source: Wellcome Trust

向作者/读者索取更多资源

The gastrointestinal hormone INSL5 stimulates the release of 5-HT from endocrine cells through the RXFP4 receptor, which in turn act on 5-HT3 receptors of enteric sensory neurons to elicit propulsive reflexes.
The gastrointestinal hormone, insulin-like peptide 5 (INSL5), is found in large intestinal enteroendocrine cells (EEC). One of its functions is to stimulate nerve circuits that increase propulsive activity of the colon through its receptor, the relaxin family peptide 4 receptor (RXFP4). To investigate the mechanisms that link INSL5 to stimulation of propulsion, we have determined the localisation of cells expressing Rxfp4 in the mouse colon, using a reporter mouse to locate cells expressing the gene. The fluorescent signal indicating the location of Rxfp4 expression was in EEC, the greatest overlap of Rxfp4-dependent labelling being with cells containing 5-HT. In fact, > 90% of 5-HT cells were positive for Rxfp4 labelling. A small proportion of cells with Rxfp4-dependent labelling was 5-HT-negative, 11-15% in the distal colon and rectum, and 35% in the proximal colon. Of these, some were identified as L-cells by immunoreactivity for oxyntomodulin. Rxfp4-dependent fluorescence was also found in a sparse population of nerve endings, where it was colocalised with CGRP. We used the RXFP4 agonist, INSL5-A13, to activate the receptor and probe the role of the 5-HT cells in which it is expressed. INSL5-A13 administered by i.p. injection to conscious mice caused an increase in colorectal propulsion that was antagonised by the 5-HT3 receptor blocker, alosetron, also given i.p. We conclude that stimuli that excite INSL5-containing colonic L-cells release INSL5 that, through RXFP4, excites 5-HT release from neighbouring endocrine cells, which in turn acts on 5-HT3 receptors of enteric sensory neurons to elicit propulsive reflexes.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.4
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据