Bi-layered carboxymethyl cellulose-collagen vitrigel dual-surface adhesion-prevention membrane

During wound regeneration, both cell adhesion and adhesion-inhibitory functions must be controlled in parallel. We developed a membrane with dual surfaces by merging the properties of carboxymethyl cellulose (CMC) and collagen using vitrification. A rigid membrane was formed by vitrification of a bi-layered CMC and collagen hydrogel without using cross-linking reagents, thus providing dual functions, strong cell adhesion-inhibition with the CMC layer, and cell adhesion with the collagen layer. We referred to this bi-layered CMC-collagen vitrigel membrane as Bi-C-CVM and optimized the process and materials. The introduction of the CMC layer conferred a tough but stably wet property to Bi-C-CVM. This enables Bi-C-CVM to cover wet tissue and make the membrane non-detachable while preventing tissue adhesion on the other side. The bi-layered vitrification procedure can expand the customizability of collagen vitrigel devices for wider medical applications.

Automated summary

During wound regeneration, a membrane with dual surfaces, combining the properties of carboxymethyl cellulose (CMC) and collagen, was developed using vitrification. This membrane provides strong cell adhesion-inhibition and cell adhesion simultaneously.