期刊
CARBOHYDRATE POLYMERS
卷 285, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2022.119256
关键词
Cassava starch; Sugarcane bagasse; Extrusion pretreatment; Enzymatic saccharification; Glucose yield; Water solubility index
资金
- CNPq (Brazilian National Council for Scientific and Technological Development) [429529/2018-0, 306619/2019-9]
- FAPERJ (Research Support Foundation for the State of Rio de Janeiro) [E-26/010.002491/2019]
- FINEP (Funding Authority of Studies and Projects) from the Brazilian Ministry of Science, Technology and Innovation [01.09.0566.001421/08]
- Japan International Cooperation Agency (JICA)
- CNPq
- TWAS (The Academy of Sciences for the Developing World) [167092/2018-9]
The enzymatic hydrolysis of native starch can be enhanced by dry extrusion pretreatment, which reduces crystallinity and improves glucose yield. The study found that extruded CS:SB (1:0.25) had the highest glucose yield and hydrolysis efficiency.
The enzymatic hydrolysis of native starch lacks efficiency because starch is mostly confined in semi-crystalline granules. To address the challenges associated with gelatinization and render native cassava starch (CS) amenable to enzymatic hydrolysis (enzyme cocktail from Aspergillus awamori and Trichoderma reesei), dryextrusion pretreatment of CS mixed with sugarcane bagasse (SB) was studied. Results showed that among the CS:SB mass ratios studied (1:1; 1:0.5 and 1:0.25), extruded CS:SB (1:0.25) gave the highest 3-hour glucose yield (71.5%) after enzymatic hydrolysis. Extrusion reduced CS:SB (1:0.25) crystallinity by 78% and increased the intensity of all major FTIR absorption bands by 67-202%. The optimum 3-hour glucose yield from extruded CS: SB (1:0.25) hydrolysis was 74.1%, which was 330% higher than from untreated CS. The water absorption and solubility indices of the treated biomass increased by 145% and 12,640%, respectively under the optimum conditions, aiding the hydrolysis process. The dry extrudates were easy to manipulate and store.
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