期刊
CARBOHYDRATE POLYMERS
卷 286, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2022.119286
关键词
Fucoidan; Fucoidanase; Enzyme-assisted extraction; Angiogenesis; Inflammation; Bone regeneration
资金
- Villum Foundation
- EU InterReg-Deutschland-Denmark (FucoSan Project Health from the Sea) [39-1.0-16]
Fucoidans, polysaccharides found in brown macroalgae, have multiple bioactivities important for bone regeneration and bone health. However, their medical applications are limited due to their chemical heterogeneity and unclear structure-function relationships. Innovations in extraction techniques and post processing steps are needed to produce homogeneous fucoidan molecules with customized bioactivities.
Fucoidans are polysaccharides from brown macroalgae, showing multiple bioactivities important for bone regeneration and bone health. However, the use of fucoidans in medical applications remains sparse due to the heterogeneity in their chemical properties and unclear structure-function relationships. Innovations in extraction techniques and post processing steps are needed to produce homogeneous fucoidan molecules with tailorable bioactivities. Here, we applied enzyme-assisted extraction coupled with enzymatic hydrolysis by Fhf1 fucoidanase to generate low (LMW) and medium molecular weight (MMW) fucoidans from Fucus evanescens. In contrast to the anti-angiogenic properties of the high molecular weight fucoidan, LMW and MMW no longer suppressed the production of pro-angiogenic molecules by bone stem cells, nor impaired the formation of prevascular structures in vitro. In contrast to LMW, a pro-inflammatory response of OEC was observed after treatment with high concentrations of MMW. Thus, fucoidanase hydrolysis could be a useful tool to tailor the bioactivity of fucoidans.
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