期刊
CARBOHYDRATE POLYMERS
卷 280, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2021.119006
关键词
Caulerpa lentillifera; Sulfated polysaccharide; COVID-19; SARA-CoV-2
资金
- National Key Research and Development Program of China [2019YFD0902005]
- National Natural Science Foundation of China [31801491]
Caulerpa lentillifera, an edible seaweed, contains a sulfated polysaccharide (CLSP-2) with potential antiviral activity against SARS-CoV-2.
Caulerpa lentillifera (Bryopsidophyceae, Chlorophyta) is an edible seaweed attracting great attention for its expansion of farming scale and increasing consumption in these years. In the present study, a sulfated polysaccharide (CLSP-2) was isolated and separated from C. lentillifera, and its chemical structure was elucidated by a series of chemical and spectroscopic methods. Among these methods, mild acid hydrolysis and photocatalytic degradation were applied to release mono- and oligo-saccharide fragments which were further identified by HPLC-MSn analysis, affording the information of the sugar sequences and the sulfate substitution in CLSP-2. Results indicated that the backbone of CLSP-2 was constructed of -> 6)-beta-Manp-(1 -> with sulfated branches at C2, which were comprised of prevalent -> 3)-beta-Galp4S-(1 ->, -> 3)-beta-Galp2,4S-(1 ->, and minor Xyl. In addition, the virus neutralization assay revealed that CLSP-2 could effectively protect HeLa cells against SARS-CoV-2 infection with an IC50 of 48.48 mu g/mL. Hence, the present study suggests CLSP-2 as a promising agent against SARS-CoV-2.
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