4.7 Article

Assessment of human microbiota stability across longitudinal samples using iteratively growing-partitioned clustering

期刊

BRIEFINGS IN BIOINFORMATICS
卷 23, 期 2, 页码 -

出版社

OXFORD UNIV PRESS
DOI: 10.1093/bib/bbac055

关键词

microbiota stability; k-means; iterative clustering; individual variability; longitudinal sampling

资金

  1. Institute of Health Carlos III(ISCIII) [CP19/00132]
  2. European Social Fund (ESF/FSE)
  3. European Regional Development Funds (ERDF/FEDER) inValencian Community

向作者/读者索取更多资源

Microbiome research is rapidly advancing, and there is a need for novel methods to assess the stability of microbiota data. The mu STASIS package provides a multifunctional framework to evaluate individual-centered microbiota stability, dealing with the sparse and compositional nature of microbiota data. It is a useful tool for assessing the stability of gut microbiota.
Microbiome research is advancing rapidly, and every new study should definitively be based on updated methods, trends and milestones in this field to avoid the wrong interpretation of results. Most human microbiota surveys rely on data captured from snapshots-single data points from subjects-and have permitted uncovering the recognized interindividual variability and major covariates of such microbial communities. Currently, changes in individualized microbiota profiles are under the spotlight to serve as robust predictors of clinical outcomes (e.g. weight loss via dietary interventions) and disease anticipation. Therefore, novel methods are needed to provide robust evaluation of longitudinal series of microbiota data with the aim of assessing intrapersonally short-term to long-term microbiota changes likely linked to health and disease states. Consequently, we developed microbiota STability ASsessment via Iterative cluStering (mu STASIS)-a multifunction R package to evaluate individual-centered microbiota stability. mu STASIS targets the recognized interindividual variability inherent to microbiota data to stress the tight relationships observed among and characteristic of longitudinal samples derived from a single individual via iteratively growing-partitioned clustering. The algorithms and functions implemented in this framework deal properly with the sparse and compositional nature of microbiota data. Moreover, the resulting metric is intuitive and independent of beta diversity distance methods and correlation coefficients, thus estimating stability for each microbiota sample rather than giving nonconsensus magnitudes that are difficult to interpret within and between datasets. Our method is freely available under GPL-3 licensing. We demonstrate its utility by assessing gut microbiota stability from three independent studies published previously with multiple longitudinal series of multivariate data and respective metadata.

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